Anti-Acanthamoeba Effects of Silver and Gold Nanoparticles and Contact Lenses Disinfection Solutions

  • Maryam NIYYATI Dept. of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Roghayeh SASANI Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Mehdi MOHEBALI Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Mahmoud GHAZI-KHANSARI Dept. of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Faranak KARGAR Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran
  • Elham HAJIALILO Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Mostafa REZAEIAN Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Keywords: Acanthamoeba, Acanthamoeba keratitis, Nanoparticles

Abstract

Background: This study aimed to investigate the anti-Acanthamoeba effects of the most used marketed disinfecting solutions in Iran. Moreover, the efficacy of some nano-compounds was tested against pathogenic Acanthamoeba.Methods: The present study was conducted in the School of Public Health, Tehran University of Medical sciences, Tehran, Iran during 2015-2016.  Cysts of Acanthamoeba T4 genotype (7 x 104 /ml) mixed at the equal volume with contact lens solutions including Opti-free, Ginza, ReNu, Maxima, Light, and Cyclean for the recommended time by the manufacturers. Nano-silver and nano-gold compounds were also treated with the amoebae. Chlorhexidine 0.02% and normal saline were used as positive and negative controls, respectively. Dead and alive amoebae were determined using vital stain and suspension was cultured in non-nutrient agar. The entire process was repeated at least three times.Results: In none of the solutions in the manufacturer's brochure recommended time, full cytotoxic effect was observed on the cysts of Acanthamoeba. Opti free express solution destroyed the cysts after 6 days. Nanosilver and nano-gold compounds showed no cytotoxic effect on the cysts of Acanthamoeba.Conclusion: None of the Nanoparticles compounds as well as contact lenses disinfecting solutions which studied was effective on Acanthamoeba cysts in the manufacturer's brochure recommended time. However, continuing study on Nano-silver and Nano-gold compounds to find effective ingredients against Acanthamoeba are highly recommended.

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Published
2018-06-25
How to Cite
1.
NIYYATI M, SASANI R, MOHEBALI M, GHAZI-KHANSARI M, KARGAR F, HAJIALILO E, REZAEIAN M. Anti-Acanthamoeba Effects of Silver and Gold Nanoparticles and Contact Lenses Disinfection Solutions. IJPA. 13(2):180-5.
Section
Original Article(s)