New Nodule Type Found in the Lungs of Pomacea canaliculata, an Intermediate Host of Angiostrongylus cantonensis

  • Yue GUO School of Medicine, Huzhou University, Huzhou, China
  • Hong Chang ZHOU School of Medicine, Huzhou University, Huzhou, China
  • Ying DONG School of Medicine, Huzhou University, Huzhou, China
  • Ting ZHANG School of Medicine, Huzhou University, Huzhou, China
  • Yu Yang SUN School of Medicine, Huzhou University, Huzhou, China
  • Jian Feng ZHONG Infectious Diseases Dept., Huzhou Central Hospital, Huzhou, China
  • Yu Liang CAO Intensive Medicine, No.98 Hospital of PLA, Huzhou, Zhejiang, China
  • Sheng Wen SHAO School of Medicine, Huzhou University, Huzhou, China
  • Yong Liang PAN School of Medicine, Huzhou University, Huzhou, China
  • Hai Yan DONG School of Medicine, Huzhou University, Huzhou, China
Keywords: Pomacea canaliculata, Lung nodule, 18S ribosomal RNA, Poterioochromonas sp, Angiostrongylus cantonensis

Abstract

Background: Pomacea canaliculata (P.canaliculata) lung nodules, were commonly caused by Angiostrongylus cantonensis infection. Here, we found a new nodule type without any parasites. Methods: Overall, 447 P. canaliculata snails were collected in Ning Bo, Zhe Jiang, China in 2018. In order to exhibit the similarities and differences between two nodules types (2018, Huzhou Zhejiang, China), both types were collected in formalin for tissue pathological sectioning. Besides, to obtain the microbial community of the new nodule, the 18S ribosomal RNA (rRNA) gene of it was amplified and analyzed using the Illumina second-generation sequencing platform. Results: Although two nodules were found in the lungs of P. canaliculata, they were different in shape and pathology. Illumina sequencing indicated Poterioochromonas sp., a species of golden algae, might be the causing agent of the new nodule. Conclusion: We firstly found a new pathological nodule type in the lungs of P. canaliculata, and this nodule might be induced by golden algae infection, however, the direct link between the golden algae and the new nodules, as well as the nodules’ impact on the snails’ physiology and A. cantonensis infection require further study.

Author Biography

Yue GUO, School of Medicine, Huzhou University, Huzhou, China
School of Medicine, Hu Zhou University, No. 759, East 2nd Road, Huzhou, Zhe Jiang, China

References

Lv, S., et al., Invasive snails and an emerging infectious disease: results from the first national survey on Angiostrongylus cantonensis in China. PLoS neglected tropical diseases, 2009. 3(2): p. e368.

Lv, S., et al., Angiostrongylus cantonensis: morphological and behavioral investigation within the freshwater snail Pomacea canaliculata. Parasitology research, 2009. 104(6): p. 1351-1359.

Tunholi-Alves, V.M., et al., Effects of infection by larvae of Angiostrongylus cantonensis (Nematoda, Metastrongylidae) on the metabolism of the experimental intermediate host Biomphalaria glabrata. Experimental parasitology, 2012. 131(2): p. 143-147.

Liu, H., et al., A comparative study of three methods in detecting Angiostrongylus cantonensis larvae in lung tissue of Pomacea canaliculata. Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi= Chinese journal of parasitology & parasitic diseases, 2007. 25(1): p. 53-56.

Liu, J., et al., Integrating microbial fuel cells with anaerobic acidification and forward osmosis membrane for enhancing bio-electricity and water recovery from low-strength wastewater. Water research, 2017. 110: p. 74-82.

Hjelmsø, M.H., et al., High-resolution melt analysis for rapid comparison of bacterial community compositions. Applied and environmental microbiology, 2014. 80(12): p. 3568-3575.

Qi, X.-Z., et al., Ammonia exposure alters the expression of immune-related and antioxidant enzymes-related genes and the gut microbial community of crucian carp (Carassius auratus). Fish & shellfish immunology, 2017. 70: p. 485-492.

Lejzerowicz, F., et al., High-throughput sequencing and morphology perform equally well for benthic monitoring of marine ecosystems. Scientific reports, 2015. 5: p. 13932.

Logares, R., et al., Diversity patterns and activity of uncultured marine heterotrophic flagellates unveiled with pyrosequencing. The ISME journal, 2012. 6(10): p. 1823.

Jünemann, S., et al., Updating benchtop sequencing performance comparison. Nature biotechnology, 2013. 31(4): p. 294.

Werner, J.J., et al., Comparison of Illumina paired-end and single-direction sequencing for microbial 16S rRNA gene amplicon surveys. The ISME journal, 2012. 6(7): p. 1273.

Sinclair, L., et al., Microbial community composition and diversity via 16S rRNA gene amplicons: evaluating the illumina platform. PloS one, 2015. 10(2): p. e0116955.

Coil, D., G. Jospin, and A.E. Darling, A5-miseq: an updated pipeline to assemble microbial genomes from Illumina MiSeq data. Bioinformatics, 2014. 31(4): p. 587-589.

Fadrosh, D.W., et al., An improved dual-indexing approach for multiplexed 16S rRNA gene sequencing on the Illumina MiSeq platform. Microbiome, 2014. 2(1): p. 6.

Bálint, M., et al., An Illumina metabarcoding pipeline for fungi. Ecology and evolution, 2014. 4(13): p. 2642-2653.

Lawley, B. and G.W. Tannock, Analysis of 16S rRNA Gene Amplicon Sequences Using the QIIME Software Package, in Oral Biology. 2017, Springer. p. 153-163.

Kõljalg, U., et al., Towards a unified paradigm for sequence‐based identification of fungi. Molecular ecology, 2013. 22(21): p. 5271-5277.

Fish, J.A., et al., FunGene: the functional gene pipeline and repository. Frontiers in microbiology, 2013. 4: p. 291.

Wang, Q., et al., Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Applied and environmental microbiology, 2007. 73(16): p. 5261-5267.

Procop, G.W., North American paragonimiasis (caused by Paragonimus kellicotti) in the context of global paragonimiasis. Clinical microbiology reviews, 2009. 22(3): p. 415-446.

ENDow, K. and S. OHTA, The symbiotic relationship between bacteria and a mesogastropod snail, Alviniconcha hessleri, collected from hydrothermal vents of the Mariana Back-Arc Basin. Bulletin of Japanese Society of Microbial Ecology, 1989. 3(2): p. 73-82.

Duval, D., et al., A novel bacterial pathogen of Biomphalaria glabrata: a potential weapon for schistosomiasis control? PLoS neglected tropical diseases, 2015. 9(2): p. e0003489.

Cole, R., C.S. Richards, and T. Popkin, Novel bacterium infecting an African snail. Journal of bacteriology, 1977. 132(3): p. 950-966.

Justine, J.-L., et al., Viral particles in Temnocephala iheringi (Platyhelminthes, Temnocephalidea), a parasite of the mollusc Pomacea canaliculata. Journal of Invertebrate Pathology, 1991. 57(2): p. 287-289.

Vega, I., et al., Facultative and obligate symbiotic associations of Pomacea canaliculata (Caenogastropoda, Ampullariidae). Biocell, 2006. 30(2): p. 367-375.

Damborenea, M., Patrones de distribución y abundancia de Temnocephala iheringi (Platyhelminthes, Temnocephalidae) en una población de Pomacea canaliculata (Mollusca Ampullariidae). Gayana, Zoología, 1996. 60: p. 1-12.

Ho, J.-S. and V.E. Thatcher, A new family of cyclopoid copepods (Ozmanidae) parasitic in the hemocoel of a snail from the Brazilian Amazon. Journal of Natural History, 1989. 23(4): p. 903-911.

Gamarra-Luques, C., et al., Intrahost distribution and trasmission of a new species of cyclopoid copepod endosymbiotic to a freshwater snail, Pomacea canaliculata (Caenogastropoda, Ampullariidae) from Argentina. Biocell, 2004. 28(2): p. 155-164.

Carpenter, E.J. and S. Janson, Intracellular cyanobacterial symbionts in the marine diatom Climacodium frauenfeldianum (Bacillariophyceae). Journal of Phycology, 2000. 36(3): p. 540-544.

Prechtl, J., et al., Intracellular spheroid bodies of Rhopalodia gibba have nitrogen-fixing apparatus of cyanobacterial origin. Molecular biology and evolution, 2004. 21(8): p. 1477-1481.

Ma, M., Y. Gong, and Q. Hu, Identification and feeding characteristics of the mixotrophic flagellate Poterioochromonas malhamensis, a microalgal predator isolated from outdoor massive Chlorella culture. Algal Research, 2018. 29: p. 142-153.

Beisser, D., et al., Quantitative proteomics reveals ecophysiological effects of light and silver stress on the mixotrophic protist poterioochromonas malhamensis. PloS one, 2017. 12(1): p. e0168183.

Cho, I. and M.J. Blaser, The human microbiome: at the interface of health and disease. Nature Reviews Genetics, 2012. 13(4): p. 260.

Gilbert, J.A., J.K. Jansson, and R. Knight, The Earth Microbiome project: successes and aspirations. BMC biology, 2014. 12(1): p. 69.

Pike, L.J., E. Viciani, and N. Kumar, Genome watch: Microbial diversity knows no borders. 2018, Nature Publishing Group.

Tragin, M., A. Zingone, and D. Vaulot, Comparison of coastal phytoplankton composition estimated from the V4 and V9 regions of the 18S rRNA gene with a focus on photosynthetic groups and especially Chlorophyta. Environmental microbiology, 2017.

Published
2018-09-23
How to Cite
1.
GUO Y, ZHOU HC, DONG Y, ZHANG T, SUN YY, ZHONG JF, CAO YL, SHAO SW, PAN YL, DONG HY. New Nodule Type Found in the Lungs of Pomacea canaliculata, an Intermediate Host of Angiostrongylus cantonensis. IJPA. 13(3):362-8.
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Original Article(s)