A Serological Survey of Cystic Echinococcosis in Equids in East of Turkey
AbstractBackground: Cystic echinococcosis (CE), caused by hydatid cysts, is a widespread and hazardous disease in humans and animals worldwide. The disease is very common in Turkey, causing serious economic losses and public health problem. In this study, the seroprevalence of equine CE was determined by enzyme-linked immunosorbent assay (ELISA).Methods: Partially purified cyst fluid antigen from sheep hydatid cyst fluid was used as antigen in ELISA. A total of 250 equids consisting of 206 donkeys and 44 horses from various regions of Erzurum province of Eastern Turkey.Results: Anti- Echinococcus granulosus antibodies were detected in 78 (31.2%) of 250 equids. The prevalence rate was 20.4% for horses and 33.5% for donkeys. There was no statistically difference between sex and ages groups for both horses and donkeys (P>0.05).Conclusion: Equine CE is quite endemic in Eastern Turkey. The high prevalence of CE suggests that equids in the transmission cycles is possible as a source of infection for definitive hosts.
Soulsby EJL. Helmints, Arthropods and Protozoa of Domesticated Animals, 7th Edition, London: Baillare Tindall, 1986.
Lightowlers MW, Rickard MD, Honey RD, Obendorf DL, Mitchell GF. Serological diagnosis of Echinococcus granulosus infection in sheep using cyst fluid antigen processed by antibody affinity chromotograph. Aust Vet J. 1984;61:101-108.
Umur S, Aslantas O. Prevalence and economic importance of hydatidosis in ruminants slaughtered in Kars municipality slaughterhouse. T Parazitol Derg. 1993;17:27-34 (in Turkish).
Simsek S, Koroglu E. Evaluation of enzyme- linked immunosorbent assay (ELISA) and enzyme-linked immunoelectrotransfer blot (EITB) for immunodiagnosis of hydatid diseases in sheep. Acta Trop. 2004;92:17-24.
Simsek S, Koroglu E, Dumanli N, Aktas M, Saki CE, Altay K, Utuk AE. Seroprevalence of cattle hydatidosis in some districts in the east Anatolian region of Turkey. Tr J Vet Anim Sci. 2005; 29:1305-1310.
Simsek S, Baklaya I, Koroglu E. Epidemiological survey and molecular characterization of Echinococcus granulosus in cattle in an endemic area of eastern Turkey. Vet Parasitol. 2010;172:347-349.
Alibasoglu A, Yalciner S. 1933-1961 yılları arasında Ankara ve yöresinde atlarda görülen hastalıklara toplu bir bakış.Ankara Univ Vet Fak Derg. 1965;12: 98-111 (in Turkish).
Gonenc B, Ayaz E, Gicik Y. The prevalence of cyst hydatid in horses and donkeys and the capability of protoscolices forming seconder cyst in mice. T Parazitol Derg. 1998;22: 428-431.
Oge S, Kircali F, Yildirim A, Oge H. Hydatidosis (Hydatid cyst) in equines. Ankara Univ Vet Fak Derg 2004;51:75-76.
Moro P, Verastagui M, Gilman RH, Falcon N, Bernal T, Gavidia C, Gonzalez A, Malqui V, Moro MH, Dueger E. Enzyme- linked immunoelectrotransfer blot assay for diagnosis of hydatidosis (Echinococcus granulosus) in sheep. Vet Rec. 1997;140:605-606.
Oriol R, Williams JF, Perez-Esandi MV, Oriol C. Purification of lipoprotein antigens of Echinococcus granulosus from sheep hydatid fluid. Am J Trop Med Hyg. 1971;20:569-574.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with pholin phenol reagent. J Biol Chem. 1951;193:265-275.
Simsek S, Risvanli A, Utuk AE, Yuksel M, Saat N, Koroglu E. Evaluation of relationship between repeat breeding and Fasciola hepatica and hydatid cyst infections in cows in Elazig district of eastern Turkey. Res Vet Sci. 2007;83:102-104.
Gundogdu C, Arslan R, Arslan MO, Gicik Y. Evaluation of cystic and alveolar Echinococcosis cases in people in Erzurum and surrounding cities. T Parazitol Derg. 2005;29: 163-166.
Varcasia A, Garippa G, Pipia AP, Scala A, Brianti E, Giannetto S, Battelli G, Poglayen G, Micagni G. Cystic echinococcosis in equids in Italy. Parasitol Res. 2008;102:815-818.
Kouam MK, Diakou A, Kanzoura V, Papadopoulos E, Gajadhar AA, Theodoropoulos G. A seroepidemiological study of exposure to Toxoplasma, Leishmania, Echinococcus and Trichinella in equids in Greece and analysis of risk factors. Vet Parasitol. 2010;170:170-175.
Azlaf R, Dakkak A. Epidemiological study of the cystic echinococcosis in Morocco. Vet Parasitol. 2006;137:83-93.
Utuk AE, Simsek S, Koroglu E, McManus DP. Molecular geneticn characterization of different isolates of Echinococcus granulosus in east and southeast regions of Turkey. Acta Trop. 2008;107:192-194.
Simsek S, Eroksuz Y. Occurrence and molecular characterization of Echinococcus granulosus in Turkish mouflon (Ovis gmelinii anatolica). Acta Trop. 2009;109:167-169.