Nanosilver Colloid Inhibits Toxoplasma gondii Tachyzoites and Bradyzoites in Vitro

  • Saeedeh SHOJAEE Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Nima FIROUZEH Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Hussein KESHAVARZ Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran
  • Sanaz JAFARPOUR AZAMI Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Mahboobeh SALIMI Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Mehdi MOHEBALI Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran
Keywords: Nanosilver, Electronmicroscopy, Tachyzoite, Bradyzoite, Toxoplasma gondii

Abstract

Background: Toxoplasma gondii, the coccidian protozoan parasite with worldwide distribution, is the agent of toxoplasmosis. The disease is life threatening in congenital form and in immunocompromised patients. The present study was carried out in 2016 to evaluate the in vitro effects of nanosilver colloid on tachyzoites and bradyzoites of T. gondii, RH and Tehran strains. Methods: Different concentrations (5, 10 , 20 ppm) of  nanosilver colloid were added to tachyzoites of T. gondii , RH strain (type I) and bradyzoites and tissue cysts of T. gondii , Tehran strain (type II) and incubated for 30, 60, 90 and 120 minutes. The mortality rates of tachyzoites and bradyzoites were evaluated by trypan blue dye and MTT assay. Then SEM carried out to show the changes between control and exposed parasites. Results: The greatest mortality rate was seen in 20 ppm concentration and after 120 minutes of exposure. By electron microscopy, the structural changes were seen in tachyzoites of RH and tissue cyst of Tehran strain in comparison with control groups. Conclusion: Nanosilver colloid was effective on both tachyzoites and bradyzoites of T. gondii, RH and Tehran strains.

References

Ramos JM, Milla A, Rodríguez JC, Padilla S, Masiá M, Gutiérrez F. Seroprevalence of Toxoplasma gondii infection among immigrant and native pregnant women in Eastern Spain. Parasitol Res. (2011) 109(5):1447-1452.

Dubey JP. Toxoplasmosis from animals and humans. 2nd edition. CRC press; (2010).

Mahmoudvand H, Ziaali N, Aghaei I, Sheibani V, Shojaee S, Keshavarz H, Shabani M. The possible association between Toxoplasma gondii infection and risk of anxiety and cognitive

disorders in BALB/c mice. Pathog Glob Health. (2015) 109 (8): 369-376.

Alipour A, Shojaee S, Mohebali M, Tehranidoost M, Abdi Masoleh F, Keshavarz H.

Toxoplasma infection in schizophrenia patients: A comparative study with control group.

Iranian J Parasitol. (2011) 6 (2): 31-37.

Mahmoudvand H, Sheibani V, Shojaee S, Mirbadie SR, Keshavarz H, Esmaeelpour K, Keyhani AR, Ziaalij N. Toxoplasma gondii infection potentiates cognitive impairments of alzheimer’s disease in the balb/c mice. J Parasitol. (2016) 102(6), 629-635.

Boyle JP , Radke JR. A history of studies that examine the interactions of Toxoplasma with its host cell: Emphasis on in vitro models. Int J Parasitol. (2009) 39 (8): 903-914.

Mui EJ, Jacobus D, Milhous WK, Schiehser G, Hsu H, Roberts CW, Kirisits MJ, MacLeod R. Triazine inhibits Toxoplasma gondii tachyzoites in vitro and in vivo. Antimicrob Agents Chemother. (2005) 49 (8): 3463- 3467.

Balazs DJ, Triandafillu K, Wood P, Chevolot Y, van Delden C, Harms H, Hollenstein C, Mathieu HJ. Inhibition of bacterial adhesion on PVC endotracheal tubes by RF-oxygen glow discharge, sodium hydroxide and silver nitrate treatments. Biomaterials. 2004; 25: 2139-51

Benn T, Cavanagh B, Hristovski K, Posner JD, Westerhoff P. The release of nanosilver from consumer products used in the home. J Environ Qual. (2010) 39(6):1875-82.

Allahverdiyev AM, Abamor ES, Bagirova M, Baydar SY, Ates SC, Kaya F, Kaya C, Rafailovich M. Investigation of antileishmanial activities of Tio2@Ag nanoparticles on biological properties of L. tropica and L. infantum parasites, in vitro. Exp Parasitol. (2013) 135(1):55-63.

Said DE, El Samad LM, Gohar YM. Validity of silver, chitosan, and curcumin nanoparticles as anti-Giardia agents. Parasitol Res. (2012) 111:545- 554.

Saad AA, Soliman MI, Azzam AM, Mostafa AB. Antiparasitic activity of silver and copper oxide nanoparticles against Entamoeba histolytica and Cryptosporidium parvum cysts. J Egypt Soc Parasitol. (2015) 45(3): 593- 602.

Ali- Heydari, S, Keshavarz, H, Shojaee, S, Mohebali M. Diagnosis ofantigenic markers of acute toxoplasmosis by IgG avidity immunoblotting. Parasite (2013) 20, 18: 1-4.

Gaafar M R, Mady RF, Diab RG, Shalaby Th I. Chitosan and silver nanoparticles: Promising anti-toxoplasma agents. Experimental Parasitol. (2014) 143: 30-38.

Kumar R, Münstedt H. Silver ion release from antimicrobial polyamide/silver composites. Biomaterials. (2005) 26:2081- 8.

Panyam J, Labhasetwar V. Biodegradable nanoparticles for drug and gene delivery to cells and tissue. Adv Drug Deliv Rev. (2003) 55(3):329- 47.

Cho KH, Park JE, Osaka T, Park SG. The study of antimicrobial activity and preservative effects of nanosilver ingredient. Electrochim Acta. (2005) 51(50): 956-960.

Willcox MDP, Hume EBH, Vijay AK, Petcavich R. Ability of silver- impregnated contact lenses to control microbial growth and colonization. J Optom. (2010) 3(3): 143- 148.

Published
2019-09-18
How to Cite
1.
SHOJAEE S, FIROUZEH N, KESHAVARZ H, JAFARPOUR AZAMI S, SALIMI M, MOHEBALI M. Nanosilver Colloid Inhibits Toxoplasma gondii Tachyzoites and Bradyzoites in Vitro. Iran J Parasitol. 14(3):362-367.
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Original Article(s)