Iranian Journal of Parasitology 2017. 12(3):364-373.

Prevalence and Risk Factors of Human Intestinal Parasites in Roudehen, Tehran Province, Iran
Nasrin HEMMATI, Elham RAZMJOU, Saeideh HASHEMI-HAFSHEJANI, Abbas MOTEVALIAN, Lameh AKHLAGHI, Ahmad Reza MEAMAR

Abstract


Background: Intestinal parasitic infections are among the most common infections and health problems worldwide. Due to the lack of epidemiologic information of such infections, the prevalence of, and the risk factors for, enteric parasites were investigated in residents of Roudehen, Tehran Province, Iran.

Methods: In this cross-sectional study, 561 triple fecal samples were collected through a two-stage cluster-sampling protocol from Jun to Dec 2014. The samples were examined by formalin-ether concentration, culture, and with molecular methods.

Results: The prevalence of enteric parasites was 32.7% (95% CI 27.3–38). Blastocystis sp. was the most common intestinal protozoan (28.4%; 95% CI 23.7–33.0). The formalin-ether concentration and culture methods detected Blastocystis sp., Entamoeba coli, Giardia intestinalis, Dientamoeba fragilis, Iodamoeba butschlii, Entamoeba complex cysts or trophozoite, Chilomastix mesnilii, and Enterobius vermicularis. Single-round PCR assay for Entamoeba complex were identified Entamoeba dispar and E. moshkovskii. E. histolytica was not observed in any specimen. Multivariate analysis showed a significant association of parasites with water source and close animal contact. There was no correlation between infections and gender, age, occupation, education, or travel history. Protozoan infections were more common than helminth infections.

Conclusion: This study revealed a high prevalence of enteric protozoan parasite infection among citizens of Rodehen. As most of the species detected are transmitted through a water-resistant cyst, public and individual education on personal hygiene should be considered to reduce transmission of intestinal parasites in the population. 


Keywords


Blastocystis, Entamoeba, Intestinal parasite, Prevalence, Protozoa

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References


Schuster H, Chiodini PL. Parasitic infections of the intestine. Curr Opin Infect Dis. 2001; 14(5):587–91.

Calderaro A, Montecchini S, Rossi S et al. Intestinal parasitoses in a tertiary-care hospital located in a non-endemic setting during 2006-2010. BMC Infect Dis. 2014; 14:264.

Fletcher S, Caprarelli G, Merif J et al. Epidemiology and geographical distribution of enteric protozoan infections in Sydney, Australia. J Public Health Res. 2014; 3(2):298.

Daryani A, Sharif M, Nasrolahei M et al. Epidemiological survey of the prevalence of intestinal parasites among schoolchildren in Sari, northern Iran. Trans R Soc Trop Med Hyg. 2012; 106(8):455–9.

Li XX, Chen JX, Wang LX et al. Prevalence and risk factors of intestinal protozoan and helminth infections among pulmonary tuberculosis patients without HIV infection in a rural county in P. R. China. Acta Trop. 2015; 149:19–26.

Rokni MB. The present status of human helminthic diseases in Iran. Ann Trop Med Parasitol. 2008; 102(4):283–95.

Haghighi A, Khorashad AS, Nazemalhosseini Mojarad E, Khorashad AS, Nazemalhosseini Mojarad E, Kazemi B, Rostami Nejad M, Rasti S. Frequency of enteric protozoan parasites among patients with gastrointestinal complaints in medical centers of Zahedan, Iran. Trans R Soc Trop Med Hyg. 2009; 103(5):452–4.

Sayyari AA, Imanzadeh F, Bagheri Yazdi SA et al. Prevalence of intestinal parasitic infections in the Islamic Republic of Iran. East Mediterr Health J. 2005; 11(3):377–83.

Solaymani-Mohammadi S, Rezaian M, Babaei Z et al. Comparison of a stool antigen detection kit and PCR for diagnosis of Entamoeba histolytica and Entamoeba dispar infections in asymptomatic cyst passers in Iran. J Clin Microbiol. 2006; 44(6):2258–61.

Kuzehkanani AB, Rezaei S, Babaei Z et al. Enteric protozoan parasites in rural areas of Bandar-Abbas, southern Iran: comparison of past and present situation. Iran J Public Health. 2011; 40(1):80–5.

Niyyati M, Rezaeian M, Zahabion F et al. A survey on intestinal parasitic infections in patients referred to a hospital in Tehran. Pak J Med Sci. 2009; 25(1):87–90.

Census. Census of the Islamic Republic of Iran, Statistical Centre of Iran 2011 [cited; Available from: http://www.amar.org.ir/

Clark CG, Diamond LS. Methods for cultivation of luminal parasitic protists of clinical importance. Clin Microbiol Rev. 2002; 15(3):329–41.

Hamzah Z, Petmitr S, Mungthin M et al. Differential detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii by a single-round PCR assay. J Clin Microbiol. 2006; 44(9):3196–200.

Arani AS, Alaghehbandan R, Akhlaghi L et al. Prevalence of intestinal parasites in a population in south of Tehran, Iran. Rev Inst Med Trop Sao Paulo. 2008; 50(3):145–9.

Boonjaraspinyo S, Boonmars T, Kaewsamut B et al. A cross-sectional study on intestinal parasitic infections in rural communities, northeast Thailand. Korean J Parasitol. 2013; 51(6):727–34.

Hooshyar H, Rezaian M, Mahmoodi M et al. A field study of the distribution of Entamoeba histolytica /dispar cyst passers in northern, central, and southern Iran. Iran J Publ Health. 2004; 33(2):28–32.

Hooshyar H, Rezaian M, Kazemi B et al. The distribution of Entamoeba histolytica and Entamoeba dispar in northern, central, and southern Iran. Parasitol Res. 2004; 94(2):96–100.

Tan KS. New insights on classification, identification, and clinical relevance of Blastocystis spp. Clin Microbiol Rev. 2008; 21(4):639–65.

Wawrzyniak I, Poirier P, Viscogliosi E et al. Blastocystis, an unrecognized parasite: an overview of pathogenesis and diagnosis. Ther Adv Infect Dis. 2013; 1(5):167–78.

Clark CG, van der Giezen M, Alfellani MA et al. Recent developments in Blastocystis research. In: Rollinson D, editor. Advances in Parasitology: Academic Press; 2013. p. 1–32.

Poirier P, Wawrzyniak I, Vivarès CP et al. New insights into Blastocystis spp.: A potential link with irritable bowel syndrome. PLoS Pathog. 2012; 8(3):e1002545.

Akhlaghi L, Shamseddin J, Meamar AR et al. Frequency of intestinal parasites in Tehran. Iran J Parasitol. 2009; 4(2):44–7.

Röser D, Simonsen J, Nielsen HV et al. Dientamoeba fragilis in Denmark: epidemiological experience derived from four years of routine real-time PCR. Eur J Clin Microbiol Infect Dis. 2013; 32(10):1303–10.

Stark D, Barratt J, Chan D et al. Dientamoeba fragilis, the neglected trichomonad of the human bowel. Clin Microbiol Rev. 2016; 29(3):553–80.

Ghazanchaei A, Shargh S, Shabani M et al. Detection of Dientamoeba fragilis in patients referred to Chaloos Medical Care Centers by nested – polymerase chain reaction (PCR) method. Afr J Biotechnol. 2012; 11(17):4079–82.

Sarafraz S, Farajnia S, Jamali J et al. Detection of Dientamoeba fragilis among diarrheal patients referred to Tabriz health care centers by nested PCR. Trop Biomed. 2013; 30(1):113–8.

Amin O. Epidemiology of Blastocystis hominis in the United States. Res J Parasitol. 2006; 1(1):1–10.

Engsbro AL, Stensvold CR, Vedel Nielsen H et al. Prevalence, incidence, and risk factors of intestinal parasites in Danish primary care patients with irritable bowel syndrome. Scand J Infect Dis. 2014; 46(3):204–9.


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