Genetic Characterization of Toxoplasma gondii from Wild Rodents in Sichuan Province, Southwestern China

  • XinLei WANG College of Basic Medicine, Dali University, Dali, Yunnan Province, China Jinci College of Shanxi Medical University, Taiyuan, Shanxi Province, China
  • Ling DONG College of Basic Medicine, Dali University, Dali, Yunnan Province, China
  • Li ZHANG College of Basic Medicine, Dali University, Dali, Yunnan Province, China
  • Yan LV College of Basic Medicine, Dali University, Dali, Yunnan Province, China
  • HaiLong LI College of Basic Medicine, Dali University, Dali, Yunnan Province, China
Keywords: Toxoplasma gondii, Genetic typing, PCR-RFLP, Wild rodents, China

Abstract

Background: Wild rodents are the intermediate hosts of Toxoplasma gondii. The distribution of genetic diversity of T. gondii in wild rodents is of importance to understand the transmission of this parasite. This study aimed to genetically characterize T. gondii isolates from wild rodents in Sichuan province, southwestern China in 2013. Methods: Genomic DNA was extracted from 10 g wild rodents’ brain samples. Semi-nested PCR and multilocous PCR-RFLP technology were performed to examine genetic diversity of T. gondii isolates as described previously. Results: Overall, 181 brain tissues of different wild rodents, including Eothenomys miletus (n=88), Crocidura attenuate (n=9), Rattus rattus sladeni (n=46), Mus musculus Linnaeus (n=6) and R. niviventer (n=32) were tested for T. gondii DNA, respectively. Six of them were positive for the T. gondii B1 gene by semi-nested PCR amplification, 4 showed complete genotyping results for all 11 polymorphic loci (SAG1, SAG2, alt. SAG2, SAG3, BTUB, GRA6, L358, PK1, C22-8, C29-2 and Apico) by PCR-RFLP, determined to represent a potential new genotype (http://toxodb.org/toxo/). Conclusion: These results documented genetic characterization of T. gondii in wild rodents from Sichuan province, and enriched the genetic diversity of T. gondii in China.

References

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Published
2019-03-10
How to Cite
1.
WANG X, DONG L, ZHANG L, LV Y, LI H. Genetic Characterization of Toxoplasma gondii from Wild Rodents in Sichuan Province, Southwestern China. Iran J Parasitol. 14(1):106-10.
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Original Article(s)