Molecular Detection and Genotyping of Intestinal Microspor-idia from Stray Dogs in Iran

  • Moein DELROBAEI Department of Internal Medicine, School of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Shahram JAMSHIDI Department of Internal Medicine, School of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Parviz SHAYAN Department of Parasitology, School of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Elahe EBRAHIMZADE Department of Parasitology, School of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Iraj ASHRAFI TAMAI Department of Microbiology and Immunology, School of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Mostafa REZAEIAN Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Hamed MIRJALALI Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Keywords: Stray dogs, Microsporidia, Enterocytozoon bieneusi, Genotyping, Iran

Abstract

Background: Microsporidia as one of the most important pathogens in veterinary and agricultural settings, have emerged in immunocompromised patients in Iran. To date, different Enterocytozoon bieneusi genotypes have been identified in humans and animals, supporting the possibility of zoonotic zoonosis transmission potential. The aim of this study was to evaluate the distribution of E. bieneusi genotypes among overpopulated stray dogs in vicinity of Tehran, the capital city of Iran. Methods: Totally, 75 stool and 75 urine samples were obtained from 75 stray dogs during the time period from Mar 2015 to Oct 2015. DNA extraction was performed on all the samples and specific fragment of small subunit ribosomal RNA gene of E. bieneusi and Encephalitozoon spp. was amplified. Furthermore, specific primers targeting the internal transcribed spacer region of E. bieneusi were applied to determine the genotype of the microorganism. Results: Microsporidia was detected in 5.3% of stool samples, while none of the urine samples was positive for microsporidia species. Overall, 440 bp fragment of E. bieneusi was amplified in all the samples and there was no amplification for Encephalitozoon spp. The results of sequencing of 410 bp fragment of internal transcribed spacer region showed that all the E. bieneusi were genotype D. Conclusion: E. bieneusi was the most prevalent microsporidian species in the stray dogs and all the positive isolates were characterized as genotype D.

References

Hirt RP, Logsdon JM, Healy B, Dorey MW, Doolittle WF, Embley TM. Microsporidia are related to Fungi: evidence from the largest subunit of RNA polymerase II and other proteins. Proceedings of the National Academy of Sciences. 1999;96(2):580-5.

Snowden KF, Shadduck JA. Microsporidia in higher vertebrates. InThe Microsporidia and Microsporidiosis. American Society of Microbiology. 1999; 393-417.

Weiss LM. Microsporidia 2003: IWOP-8. The Journal of eukaryotic microbiology. 2003;50(6):566-8.

Cali A, Owen RL. Microsporidiosis. InLaboratory Diagnosis of Infectious Diseases. 1988;929-950. Springer New York.

Frixione E, Ruiz L, Cerbon J, Undeen AH. Germination of Nosema algerae (Microspora) spores: conditional inhibition by D2O, ethanol and Hg2+ suggests dependence of water influx upon membrane hydration and specific transmembrane pathways. Journal of Eukaryotic Microbiology. 1997;44(2):109-16.

Deplazes P, Mathis A, Muller C, Weber R. Molecular epidemiology of Encephalitozoon cuniculi and first detection of Enterocytozoon bieneusi in faecal samples of pigs. Journal of Eukaryotic Microbiology. 1996;43(5).

Liguory O, David F, Sarfati C, Derouin F, Molina JM. Determination of Types of Enterocytozoon bieneusi Strains Isolated from Patients with Intestinal Microsporidiosis. Journal of clinical microbiology. 1998;36(7):1882-5.

Mansfield KG, Carville A, Shvetz D, MacKey J, Tzipori S, Lackner AA. Identification of an Enterocytozoon bieneusi-like microsporidian parasite in simian-immunodeficiency-virus-inoculated macaques with hepatobiliary disease. The American journal of pathology. 1997;150(4):1395.

Desportes I, Charpentier YL, Galian A, Bernard F, Cochand‐Priollet B, Lavergne A, Ravisse P, Modigliani R. Occurrence of a new microsporidan: Enterocytozoon bieneusi ng, n. sp., in the enterocytes of a human patient with AIDS. Journal of Eukaryotic Microbiology. 1985;32(2):250-4.

Santín M, Fayer R. Microsporidiosis: Enterocytozoon bieneusi in domesticated and wild animals. Research in veterinary science. 2011;90(3):363-71.

Didier ES, Weiss LM. Microsporidiosis: not just in AIDS patients. Current opinion in infectious diseases. 2011;24(5):490.

Graczyk TK, Sunderland D, Rule AM, da Silva AJ, Moura IN, Tamang L, Girouard AS, Schwab KJ, Breysse PN. Urban feral pigeons (Columba livia) as a source for air-and waterborne contamination with Enterocytozoon bieneusi spores. Applied and environmental microbiology. 2007;73(13):4357-8.

Fayer R, Santin‐Duran M. Epidemiology of microsporidia in human infections. Microsporidia: Pathogens of Opportunity, First Edition. 2014;1:135-64.

Dengjel B, Zahler M, Hermanns W, Heinritzi K, Spillmann T, Thomschke A, Löscher T, Gothe R, Rinder H. Zoonotic potential of Enterocytozoon bieneusi. Journal of Clinical Microbiology. 2001;39(12):4495-9.

Leelayoova S, Subrungruang I, Rangsin R, Chavalitshewinkoon-Petmitr P, Worapong J, Naaglor T, Mungthin M. Transmission of Enterocytozoon bieneusi genotype a in a Thai orphanage. The American journal of tropical medicine and hygiene. 2005;73(1):104-7.

Jamshidi S, Tabrizi AS, Bahrami M, Momtaz H. Microsporidia in household dogs and cats in Iran; a zoonotic concern. Veterinary parasitology. 2012;185(2):121-3.

Pirestani M, Sadraei J, Forouzandeh M. Molecular characterization and genotyping of human related microsporidia in free-ranging and captive pigeons of Tehran, Iran. Infection, Genetics and Evolution. 2013;20:495-9.

Agholi M, Hatam GR, Motazedian MH. HIV/AIDS-associated opportunistic protozoal diarrhea. AIDS research and human retroviruses. 2013;29(1):35-41.

Agholi M, Hatam GR, Motazedian MH. Microsporidia and coccidia as causes of persistence diarrhea among liver transplant children: incidence rate and species/genotypes. The Pediatric infectious disease journal. 2013;32(2):185-7.

Mirjalali H, Mohebali M, Mirhendi H, Gholami R, Keshavarz H, Meamar AR, Rezaeian M. Emerging Intestinal Microsporidia Infection in HIV+/AIDS Patients in Iran: Microscopic and Molecular Detection. Iranian journal of parasitology. 2014;9(2):149.

Askari Z, Mirjalali H, Mohebali M, Zarei Z, Shojaei S, Rezaeian T, Rezaeian M. Molecular detection and identification of zoonotic microsporidia spore in fecal samples of some animals with close-contact to human. Iranian journal of parasitology. 2015 Jul;10(3):381.

Mirjalali H, Mirhendi H, Meamar AR, Mohebali M, Askari Z, Mirsamadi ES, Rezaeian M. Genotyping and molecular analysis of Enterocytozoon bieneusi isolated from immunocompromised patients in Iran. Infection, Genetics and Evolution. 2015;36:244-9.

Mathis A, Weber R, Deplazes P. Zoonotic potential of the microsporidia. Clinical Microbiology Reviews. 2005;18(3):423-45.

Mathis A, Breitenmoser AC, Deplazes P. Detection of new Enterocytozoon genotypes in faecal samples of farm dogs and a cat. Parasite. 1999;6(2):189-93.

Santín M, Vecino JA, Fayer R. Enterocytozoon bieneusi genotypes in dogs in Bogota, Colombia. The American journal of tropical medicine and hygiene. 2008;79(2):215-7.

Lores B, Del Aguila C, Arias C. Enterocytozoon bieneusi (microsporidia) in faecal samples from domestic animals from Galicia, Spain. Memórias do Instituto Oswaldo Cruz. 2002;97(7):941-5.

Zhang X, Wang Z, Su Y, Liang X, Sun X, Peng S, Lu H, Jiang N, Yin J, Xiang M, Chen Q. Identification and genotyping of Enterocytozoon bieneusi in China. Journal of clinical microbiology. 2011;49(5):2006-8.

Niichiro AB, Kimata I, Iseki M. Molecular evidence of Enterocytozoon bieneusi in Japan. Journal of Veterinary Medical Science. 2009;71(2):217-9.

Chabchoub N, Abdelmalek R, Breton J, Kanoun F, Thellier M, Bouratbine A, Aoun K. Genotype identification of Enterocytozoon bieneusi isolates from stool samples of HIV-infected Tunisian patients. Parasite: journal de la Société Française de Parasitologie. 2012;19(2):147.

Ojuromi OT, Izquierdo F, Fenoy S, Fagbenro-Beyioku A, Oyibo W, Akanmu A, Odunukwe N, Henriques-Gil N, del Aguila C. Identification and characterization of microsporidia from fecal samples of HIV-positive patients from Lagos, Nigeria. PLoS One. 2012;7(4):e35239.

Lobo ML, Xiao L, Cama V, Stevens T, Antunes F, Matos O. Genotypes of Enterocytozoon bieneusi in mammals in Portugal. Journal of Eukaryotic Microbiology. 2006;53(s1).

Cama VA, Pearson J, Cabrera L, Pacheco L, Gilman R, Meyer S, Ortega Y, Xiao L. Transmission of Enterocytozoon bieneusi between a child and guinea pigs. Journal of clinical microbiology. 2007;45(8):2708-10.

Breitenmoser AC, Mathis A, Bürgi E, Weber R, Deplazes P,. High prevalence of Enterocytozoon bieneusi in swine with four genotypes that differ from those identified in humans. Parasitology. 1999; 118: 447–453.

Published
2019-03-11
How to Cite
1.
DELROBAEI M, JAMSHIDI S, SHAYAN P, EBRAHIMZADE E, ASHRAFI TAMAI I, REZAEIAN M, MIRJALALI H. Molecular Detection and Genotyping of Intestinal Microspor-idia from Stray Dogs in Iran. Iran J Parasitol. 14(1):159-166.
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Short Communication(s)