Clinical and Molecular Evaluation of a Case of Giant Primary Splenic Hydatid Cyst: A Case Report
AbstractSpleen is an unusual location for hydatid cyst. Here we report a case of primary splenic hydatid cyst in a 41-yr-old Iranian woman from Yasuj, southwest of Iran. The patient had been admitted to Shahid Beheshti Hospital because of abdominal pain. Abdominal sonography revealed a hypoechoic lesion of 150 X 130 mm in the spleen, suggestive of hydatid cyst. Splenectomy was performed for the patient and surgical interventions revealed a hydatid cyst occupying most of splenic parenchyma. She was discharged on the 5 day of her operation. Postoperative diagnosis and confirmation of hydatid cyst was done by histopathological, molecular and serological approaches. Histopathological evaluation revealed the classical laminated layer of hydatid cyst. DNA was extracted from a part of cyst and PCR amplified. Sequencing and analysis of PCR product revealed that the isolate has the most similarity with G1 strain of Echinococcus granulosus. Patient’s serum was positive for IgG anti-hydatid cyst antibodies, using antigen-B ELISA.
Eckert J, Deplazes P. Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern. Clin Microbiol Rev. 2004; 17(1):107-135.
Sarkari B, Sadjjadi SM, Beheshtian MM, Aghaee M, Sedaghat F. Human cystic echinococcosis in Yasuj District in Southwest of Iran: an epidemiological study of seroprevalence and surgical cases over a ten-year period. Zoonoses Public Health. 2010; 57(2):146-150.
McManus DP, Zhang W, Li J, Bartley PB. Echinococcosis. Lancet. 2003; 362(9392): 1295-1304.
Geramizadeh B. Unusual locations of the hydatid cyst: a review from Iran. Iran J Med Sci. 2013; 38(1):2-14.
Sachar S, Goyal S, Goyal S, Sangwan S. Uncommon locations and presentations of hydatid cyst. Ann Med Health Sci Res. 2014; 4(3):447-452.
McManus DP. Current status of the genetics and molecular taxonomy of Echinococcus species. Parasitology. 2013; 140(13):1617-1623.
Sadjjadi SM, Abidi H, Sarkari B, Izadpanah A, Kazemian S. Evaluation of enzyme linked immunosorbent assay, utilizing native antigen B for serodiagnosis of human hydatidosis. Iran J Immunol. 2007; 4(3):167-172.
Rostami Nejad M, Nazemalhosseini Mojarad E, Nochi Z, Fasihi Harandi M, Cheraghipour K, Mowlavi GR, Zali MR. Echinococcus granulosus strain differentiation in Iran based on sequence heterogeneity in the mitochondrial 12S rRNA gene. J Helminthol. 2008;82(4):343-347.
Mohammadzadeh T, Sako Y, Sadjjadi SM, Sarkari B, Ito A. Comparison of the usefulness of hydatid cyst fluid, native antigen B and recombinant antigen B8/1 for serological diagnosis of cystic echinococcosis. Trans R Soc Trop Med Hyg. 2012; 106(6):371-375.
Rahimi H, Sadjjadi S, Sarkari B. Performance of antigen B isolated from different hosts and cyst locations in diagnosis of cystic echinococcosis. Iran J Parasitol. 2011; 6(1):12-19.
Sadjjadi SM, Sedaghat F, Hosseini SV, Sarkari B. Serum antigen and antibody detection in echinococcosis: application in serodiagnosis of human hydatidosis. Korean J Parasitol. 2009; 47(2):153-157.
Sedaghat F, Sadjjadi SM, Hosseini SV, Kazemian S, Sarkari B. Evaluation of a simple Dot-ELISA in comparison with countercurrent imm-unoelectrophoresis for diagnosis of human hydatidosis. Clin Lab. 2011; 57(3-4):201-205.
Sarkari B, Rezaei Z. Immunodiagnosis of human hydatid disease: Where do we stand? World J Methodol. 2015; 5(4):185-195.
Sarkari B, Sadjjadi SM, Abidi H, Izadpanah A, Kazemian S, Rafati A. Application of western blotting using native antigen B for serodiagnosis of human cystic echinococcosis. Iran J Parasitol. 2007; 2(3):7-12.
Fernandez-Ruiz M, Guerra-Vales JM, Enguita-Valls AB, Vila-Santos J, Garcia-Borda FJ, Morales-Gutierrez C. Splenic hydatid cyst, a rare location of extrahepatic echinococcosis: Report of six cases. Eur J Intern Med. 2008; 19(7):e51-53.
Vezakis A, Dellaportas D, Polymeneas G, Tasoulis MK, Chondrogiannis C, Melemeni A, Polydorou A, Fragulidis GP. Two cases of primary splenic hydatid cyst in Greece. Korean J Parasitol. 2012; 50(2):147-150.
Ran B, Shao Y, Yimiti Y, Aji T, Shayiding P, Jiang T, Li H, Li J, Zhang W, Wen H. Spleen-preserving surgery is effective for the treatment of spleen cystic echinococcosis. Int J Infect Dis. 2014; 29:181-183.
Sarkari B, Mansouri M, Khabisi SA, Mowlavi G. Molecular characterization and seroprevalence of Echinococcus granulosus in wild boars (Sus scrofa) in south-western Iran. Ann Parasitol. 2015;61(4):269-273.
Mansouri M, Sarkari B, Mowlavi GR. Helminth parasites of wild boars, Sus scrofa, in Bushehr Province, Southwestern Iran. Iran J Parasitol. 2016; 11(3):377-382.
Nikmanesh B, Mirhendi H, Ghalavand Z, Alebouyeh M, Sharbatkhori M, Kia E, Mohebali M, Eghbali M, Rokni MB. Genotyping of Echinococcus granulosus isolates from human clinical samples based on sequencing of mitochondrial genes in Iran, Tehran. Iran J Parasitol. 2014; 9(1):20-27.