Iranian Journal of Parasitology 2016. 11(4):542-548.

Antigenic Cross-reactivity among Haemonchus contortus, Oesophagostomum columbianum and Trichuris ovis of Goat
Ruma JAS, Joydeb GHOSH, Kinsuk DAS


Background: Cross antigenicity is the major problem in developing a reliable tool for immunodiagnosis and immunoprophylaxis of parasitic diseases. Mixed infection due to different types of gastrointestinal parasites is more common than single species infection under field condition.

Methods: The present study was undertaken to detect antigenic cross-reactivity among Haemonchus contortus, Oesophagostomum columbianum and Trichuris ovis of goats by SDS-PAGE and western blot analysis using hyperimmune sera (HIS) rose in rabbit separately against the antigens of the three nematode species.

Results: Thirteen, 16 and 14 polypeptides in crude somatic antigen (CSAg) of H. contortus (CSAg-Hc), O. columbianum (CSAg-Oc) and T. ovis (CSAg-To), respectively, were resolved in SDS PAGE analyses. It was revealed that 54 kDa peptide was shared by H.contortus and O. columbianum, whereas 47 kDa peptide was shared by O. columbianum and T. ovis. Western blot analyses revealed that three immunogenic polypeptides (MW 54, 49 and 42 kDa) in CSAg-Hc, five in CSAg-Oc (54, 47, 44, 38 and 35.5 kDa) and CSAg-To and five polypeptides (90, 51, 47, 39.5 and 31 kDa) in CSAg-To cross-reacted with the heterologous HIS. Four species-specific immunoreactive polypeptides (92, 85, 65 and 39 kDa) of H. contortus and two (72 & 26 kDa) in O. columbianum were also identified in the study. 

Conclusion: The shared polypeptides and species-specific polypeptides might be evaluated as protective antigen and subsequently exploitation for developing immunodiagnostic and for immunoprophylactic tools of for these common nematode species. 


Haemonchus contortus; Oesophagostomum colum-bianum; Trichuris ovis; Antigenic profiles; Cross-reactivity

Full Text:



Saha SB, Pramanik S, Mukherjee GS.Prevalence of gastrointestinal nematodes of goats in West Bengal. Ind J Anim Sci.1996; 11(1): 51 – 52.

Jas R, Ghosh JD. Seasonal qualitative and quantitative variation in environmental contamination with gastrointestinal nematodes of goat. Environ Ecol. 2007; 25S(4): 1142 – 1145.

Blanchard JL, Westcott RB. Enhancement of resistance of lambs to Haemonchus contortus by previous infection with Ostertagia circumcincta. Am J Vet Res.1985;46: 2136 – 2140.

Adams DB, Anderson BH, Windon RG. Cross immunity between Haemonchus contortus and Trichostrongylus colubriformis in sheep. Int J Parasitol. 1989; 19: 712 – 722.

Hendrikx WNL. De nematode Haemonchuscontortus antigeenkarakter en immuunresponsbijKonijnen en schapen.Tijdschr Diergeneesks, 1990; 115: 1092 – 1101.

Gamble HR, Purcell JP, Fetterer RH. Biochemical characterization of cuticle polypeptides from the infective larvae of Haemonchus. Comp Biochem Physiol. 1990; 96B: 421 – 429.

Canals A, Gasbarre LC. Ostertagia Ostertagi: isolation and partial characterization of somatic and metabolic antigens. Int J Parasitol. 1990; 20 (8): 1047 – 1054.

Jasmer DP, McGuire TC. Protective immunity to a blood feeding nematode (Haemonchus contortus) induced by parasite gut antigens. Infec Immun. 1991; 59 (12): 4412 – 4417.

Jasmer DP, Perryman LE, Conder GA, Crow S, McGuire T.C. Protective immunity to Haemonchus contortus induced by immunoaffinity isolated antigens that share a phylogenetically conserved carbohydrate gut surface epitope. J Immunol.1993; 151: 5450 – 5460.

Jas R, Ghosh JD, DAS K. Diagnosis of Oesophagostomum columbianum infection in goat by indirect enzyme linked immunosorbent assay. Helminthologia.2010a; 47(2): 83 – 87.

Klesius PH, Washburn SM, Haynes TB. Serum antibody response to soluble extract of the third larval stage of Ostertagia ostertagi in cattle. Vet Parasitol. 1986; 20: 307 – 314.

Lowry OH, Rosebrough NJ, Farr AB, Randall RJ. Protein measurement with the Folin-phenol reagent. J Biol Chem. 1951; 193: 265-75.

Hudson L, Hay FC. Practical Immunology.3rd ed. Blackwell scientific Publications, Oxford: London;1989.

Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature.1970; 227: 680 – 685.

Towbin H, Stachelin T, Gorden G. Elecrophoretic transfer of proteins from polyacrilamide gels to nitrocellulose sheets: Procedure and some application. Proceedings of the National Academy of Sciences, USA.1979; 76: 4350 – 4354.

Parkhouse RME, Almond NM, Cabrera Z, Harnett W. Nematode antigen in protection, diagnosis and pathology. Vet Immunol Immunopathol. 1987; 17: 313 – 324.

Schallig HDFH, Hornok S, Cornelissen JBWJ. Comparison of two enzyme immunoassays for the detection of Haemonchus contortus infections in sheep. Vet Parasitol. 1995; 57: 329 – 338.

Soulsby EJL. Helminths, arthropods and protozoa of domesticated animals. 7thed. The English Language Book Society and Bailliere, Tindall: London; 1982.

Jas R, Ghosh JD, DAS K. Polyclonal antibody based coproantigen detection immunoassay for diagnosis of Oesophagostomum columbianum infection in goats. Vet Parasitol. 2010b; 170: 262 – 267.


  • There are currently no refbacks.

Creative Commons Attribution-NonCommercial 3.0

This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.