Echinococcus granulosus Sensu Stricto in Dogs and Jackals from Caspian Sea Region, Northern Iran

  • Shirzad GHOLAMI Department of Parasitology & Mycology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  • Hefzallah JAHANDAR Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran
  • Mahdi ABASTABAR Invasive Fungi Research Center, Mazandaran University of Medical Sciences, Sari, Iran
  • Abdolsatar PAGHEH Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran
  • Iraj MOBEDI Department of Parasitology & Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Mitra SHARBATKHORI Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran Department of Parasitology & Mycology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
Keywords: Echinococcus granulosus, Genotyping, Dog, Jackal, cox1, Iran

Abstract

Background: The aim of the present study was genotyping of Echinococcus granulosus isolates from dogs and jackals in Mazandaran Province, northern Iran, and using partial sequence of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1).Methods: E. granulosus isolates (n = 15) were collected from 42 stray dogs and 16 jackals found in south of the Caspian Sea in northern Iran. After morphological study, the isolates were genetically characterized using consensus sequences (366bp) of the cox1 gene. Phylogenetic analysis of cox1 nucleotide sequence data was performed using a Bayesian Inference approach.Results: Four different sequences were observed among the isolates. Two genotypes [G1 (66.7%) and G3 (33.3%)] were identified among the isolates. The G1 sequences indicated three sequence profiles. One profile (Maz1) had 100% homology with reference sequence (AN: KP339045). Two other profiles, designated Maz2 and Maz3, had 99% homology with the G1 genotype (ANs: KP339046 and KP339047). A G3 sequence designated Maz4 showed 100% homology with a G3 reference sequence (AN: KP339048).Conclusion: The occurrence of the G1 genotype of E. granulosus sensu stricto as a frequent genotype in dogs is emphasized. This study established the first molecular characterization of E. granulosus in the province.

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Published
2016-12-21
How to Cite
1.
GHOLAMI S, JAHANDAR H, ABASTABAR M, PAGHEH A, MOBEDI I, SHARBATKHORI M. Echinococcus granulosus Sensu Stricto in Dogs and Jackals from Caspian Sea Region, Northern Iran. IJPA. 11(2):186-94.
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Original Article(s)