Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 10 4 2015 12 14 617 624 EN Farzaneh ZAHABIUN Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Seyed Mahmoud SADJJADI Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran AND Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Farideh ESFANDIARI Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran 2015 12 14 2015 12 14 Background: : Permanent slide preparation of nematodes especially small ones is time consuming, difficult and they become scarious margins. Regarding this prob­lem, a modified double glass mounting method was developed and com­pared with classic method. Methods: A total of 209 nematode samples from human and animal origin were fixed and stained with Formaldehyde Alcohol Azocarmine Lactophenol (FAAL) followed by double glass mounting and classic dehydration method us­ing Canada balsam as their mounting media. The slides were evaluated in differ­ent dates and times, more than four years. Different photos were made with differ­ent magnification during the evaluation time. Results: The double glass mounting method was stable during this time and compa­rable with classic method. There were no changes in morphologic struc­tures of nematodes using double glass mounting method with well-defined and clear differentiation between different organs of nematodes in this method. Conclusion: Using this method is cost effective and fast for mounting of small nematodes comparing to classic method.  https://ijpa.tums.ac.ir/index.php/ijpa/article/view/663 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/663/544