Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 8 1 2013 03 15 33 39 EN F Chen The Faculty of Life Sciences, Hubei University, 368 Youyi Road, Wuchang, Wuhan 430062, China Q Liu Hainan Provincial Key Laboratory of Tropical Medicine, Pharmacy School, Hainan Medical College, Haikou 571199, China Y Xue Lab of Medical Engineering, College of Medical Technology and Engineering, Henan University of Science and Techology, Luoyang 471003, China Yh Huang Hainan Provincial Key Laboratory of Tropical Medicine, Pharmacy School, Hainan Medical College, Haikou 571199, China Fy Huang Hainan Provincial Key Laboratory of Tropical Medicine, Pharmacy School, Hainan Medical College, Haikou 571199, China Y Lin Hainan Provincial Key Laboratory of Tropical Medicine, Pharmacy School, Hainan Medical College, Haikou 571199, China Gh Tan Hainan Provincial Key Laboratory of Tropical Medicine, Pharmacy School, Hainan Medical College, Haikou 571199, China J Zhou Wuhan Tuberculosis Dispensary, 28 Baofeng Road, Qiaokou, Wuhan, 430030, China Fan Chen and Qiang Liu are co-primary authors 2015 10 14   Background: Malaria remains a serious public health problem with significant morbidity and mortal-ity. This study was conducted to identify whether ficolin-A could play an active role of against mala-ria infection. Methods: The function of ficolin-A was analyzed in mouse model. The open reading frame of fico-lin-A was cloned from the liver of new born C57BL/6 mice by RT-PCR and then inserted into the expression vector of eukaryon to construct pVAX1-ficolin-A plasmid. Meanwhile, the open reading frame of the 19-kDa fragment of merozoite surface protein-1 of Plasmodium berghei (MSP119) was cloned and then the expression vector of eukaryon, pVAX1- MSP119 was constructed. Both recom-binant vectors were used in the mouse model of infection by Plasmodium berghei. Results: pVAX1-ficolin-A alone could not significantly suppress parasite density and prolong sur-vival time of infection mice; however, when injected pVAX1-ficolin-A and pVAX1- MSP119 together, the percent of invasion by Plasmodium was decreased (from 43.78% to 22.23% at 10 day after infec-tion, compared to vector ) and the survival time was prolonged significantly in the infection mouse model (P=0.01). Conclusion: Ficolin-A can enhance the immunoprotection of MSP119, it implies ficolin-A may be used as immunoenhancer in the study of vaccine defending malaria. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/529 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/529/385