Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 21 3 2026 09 01 335 347 Bahareh Bahadori Mazidi School of Paramedical Sciences, Darab, Shiraz University of Medical Sciences, Darab, Iran Zabihullah Fasehi Department of Paraclinic, Faculty of Veterinary Science, Kunduz University, Kunduz province, Afghanistan Hamed Abdollahi Department of Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Khuzestan, Iran Mehrnoosh Naderizadeh Faculty of Veterinary Medicine, Islamic Azad University Science and Research Branch, Tehran, Iran Alireza Najafi D.V.M. Graduated, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran Shadan Ghiabi Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran Ali Asghari Department of Basic Medical Sciences, Khoy University of Medical Sciences, Khoy, Iran Mohammad Fathollahzadeh Liver and Gastrointestinal Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Hamidreza Majidiani Department of Basic Medical Sciences, Faculty of Medicine, Neyshabur University of Medical Sciences, Neyshabur, Iran Leyli Shabani Faculty of Veterinary Medicine, Semnan University, Semnan, Iran 2025 12 03 2026 09 01 Background: Vaccination represents a promising strategy for preventing hydatidosis. We aimed to characterize the physicochemical properties of adenylate kinase 8 from Echinococcus granulosus (EgADK8) and identify potential B- and T-cell epitopes as vaccine candidates. Methods: Multiple web-based servers were employed to predict immunogenic epitopes and evaluate the physicochemical, antigenic, structural, and subcellular characteristics of EgADK8. Results: EgADK8 was predicted to be an extracellular protein with a molecular weight of 38.78 kDa. The protein showed no allergenic potential and exhibited high hydrophilicity, predicted thermal stability, and 36 potential post-translational modification sites. The secondary structure was mainly composed of alpha helices and random coils. The highest-quality three-dimensional model generated by the Robetta server achieved a confidence score of 0.79. Three independent prediction servers identified 16 linear and 9 conformational B-cell epitopes. Furthermore, two helper T lymphocyte (HTL) epitopes with predicted IFN-γ-inducing potential were identified after comprehensive screening. Additionally, five immunogenic human cytotoxic T lymphocyte (CTL) epitopes with predicted IFN-γ-inducing potential and three murine CTL epitopes were identified. Conclusion: These findings provide preliminary computational evidence supporting further investigation of EgADK8 as a potential component of a multiepitope vaccine against CE. The main limitation of this study was its reliance on computational analyses. Therefore, experimental validation using the whole protein and/or selected epitopes, either individually or in combination with other antigens, is required. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/4833 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/4833/1509