Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 20 4 2025 12 07 500 509 Sarwin Sultan Muhamad Mizuri 1. Department of Biology, College of Science, University of Zakho, Duhok, Iraq 2. Biology Research Center, Research Center, University of Zakho, Duhok, Iraq Wijdan Mohammed Salih Mero 1. Department of Biology, College of Science, University of Zakho, Duhok, Iraq 2. Department of Medical Microbiology, College of Science, Knowledge University, Erbil, Iraq Haval Mohammed Khalid 1. Department of Biology, College of Science, University of Zakho, Duhok, Iraq 2. Biology Research Center, Research Center, University of Zakho, Duhok, Iraq 2025 06 02 2025 12 06 Background: The study aimed to investigate the association between cytokine profiles and toxoplasmosis in newly married couples, with identifying the immunological markers to minimizing fertility complications through early detection and intervention. Methods: From May 2024 to February 2025, blood samples of 480 newlywed couples in Zakho City, Iraq, were evaluated for Anti-Toxoplasma G (IgG) and M (IgM) antibodies, as well as serum cytokines like Interleukin 12 (IL-12), Tumer Necrosis Factor α (TNF-α), Interleukin10 (IL-10), and Transforming Growth Factor ß1 (TGFß1), using ELISA. Additionally, C Reactive protein (CRP) and Erythrocyte Sedimentation Rate (ESR) were also estimated. Results: The overall seroprevalence of Anti-Toxoplasma antibodies were 25.8% (124/480), constituting 18.2% (88/480) for IgG, and 7.5% (36/480) for IgM, with highly significant difference (P< 0.001). Rates were higher in males, with age-related differences observed for both IgG and IgM. While statistically significant differences were reported for IgM alone relates to gender. Regarding cytokines, seropositive individuals showed significantly higher IL-10 and IL-12 levels than controls: IL-10 (IgG: 85.88±9.67 vs 37.88±5.06; IgM: 80.95±13.19 vs 51.72±6.19 pg/mL) and IL-12 (IgG: 22.43±3.05 vs 10.08±1.03; IgM: 29.25±5.46 vs 11.83±1.06 pg/mL). Besides, TNF-α elevated in the IgM group only. Conclusion: Elevated IL-10 and IL-12 levels in seropositive individuals suggest active immune modulation, while TNF-α dynamics point to stage-specific responses, higher in acute (IgM) infections. These immune patterns may influence reproductive health, underscoring the importance of early diagnosis and immune monitoring to reduce potential fertility complications, particularly in early marriage. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/4627 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/4627/1458