Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 20 1 2025 02 27 130 139 EN Ehsan Javanmard 1. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2. Student Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran Hanieh Mohammad Rahimi Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran Ali Taghipour 1. Department of Parasitology and Mycology, Jahrom University of Medical Sciences, Jahrom, Iran 2. Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran Sara Nemati Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mehdi Mohebali 1. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2. Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran Mostafa Rezaeian Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Ali Badrifar Division of Avian Diseases, Islamic Azad University, Shoushtar Branch, Khuzestan, Iran Elham Kazemirad Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Hamed Mirjalali Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2025 01 18 2025 02 06 Background: We aimed to investigate the prevalence of Enterocytozoon bieneusi and the circulating genotypes in wild boar in the western regions of Iran. Methods: Fifty-two fecal samples were collected from wild boars in Lorestan province, Iran. After DNA extraction, the presence of E. bieneusi was evaluated by real-time PCR. A nested PCR targeting the internal transcribed region (ITS) was employed to characterize genotypes. The PCR products were sequenced, and the genetic diversity and relationships among the genotypes were identified using MEGA X and DnaSp (V5) software. Results: Sixteen (30%) samples were positive for E. bieneusi using real-time PCR, and 11 (21%) were positive for nested PCR, which was sequenced. All 11 positive samples were identified as the BEB6 genotype (Group 2). The haplotype diversity was noted to be 0.182, and the nucleotide diversity, calculated using DnaSp, was 0. 00085. Conclusion: The findings highlight the high prevalence of the genotype BEB6 in wild boars. The presence of this genotype suggests the circulation of E. bieneusi between domesticated animals and wild boars in Iran. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/4499 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/4499/1416