Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 20 1 2025 02 26 83 90 Yahya Bayraktar Aksaray Training and Research Hospital, General Surgery Department, Aksaray, Turkey Mehmet Eryılmaz Department of General Surgery, University of Health Science, Konya City Hospital, Konya, Turkey Mehmet Ulutaş Department of General Surgery, University of Health Science, Gaziantep City Hospital, Gaziantep, Turkey Alpaslan Şahin Department of General Surgery, University of Health Science, Konya City Hospital, Konya, Turkey Gürcan Şimşek Department of General Surgery, University of Health Science, Konya City Hospital, Konya, Turkey Şerife Yüksekkaya Department of Microbiology, University of Health Science, Konya City Hospital, Konya, Turkey 2024 12 16 2025 02 26 Background: Approximately 25% of hepatic hydatid cysts rupture into the biliary tract. The precise effect of bile within the cyst on protoscoleces remains unclear. We aimed to elucidate the effect of bile on protoscoleces. Methods: The contents of hydatid cysts from the livers of three sheep were aspirated under sterile conditions. The aspirated contents were divided into 50 separate Eppendorf tubes (5 cc). Samples from each tube were stained with 0.1% eosin Y. Pink stained protoscoleces were considered dead under light microscopy (x100). A total of 100 protoscoleces were counted in each sample, and the number of live and dead protoscoleces was recorded. The tubes were randomly divided into five groups. Group 1 served as the control, Group 2 received normal saline (NS), Group 3, received hypertonic saline, Group 4 received bile, and Group 4 received diluted bile. The number of live and dead protoscoleces was recorded at the end of the first and second hours. Results: Compared to the initial count of live protoscoleces, the number of live protoscoleces increased at hours 1 and 2 in Groups 2 and 4. No live protoscoleces remained at hours 1 and 2 in Group 3. There was no significant change in Group 5. When compared to the control group, a significant increase in viability was observed only in Group 4 (P=0.001). Conclusion: Bile of sheep does not exhibit scolicidal effects; rather, it positively affects protoscoleces by increasing viability. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/4483 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/4483/1411