Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 20 3 2025 09 01 378 388 Afsaneh Khodadadi Department of Pharmaceutical Sciences, Islamic Azad University, Tehran, Iran Mohammad Hosein Rastgoo Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran Elaheh Ghiyasipour Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Reza Moradmandpaeinroudposhti Department of Veterinary Science, Faculty of Veterinary Medicine, Islamic Azad University, Tehran, Iran Mohammad Arad Zandieh Faculty of Veterinary Medicine, Islamic Azad University, Science and Research Branch, Tehran, Iran Fatemeh Zahirinia Department of Veterinary Clinical Sciences, Urmia University, Urmia, Iran Mahdi Haghdadijoo Faculty of Veterinary Medicine, Islamic Azad University, Garmsar Branch, Garmsar, Iran Ali Esfandani Faculty of Veterinary Medicine, Islamic Azad University, Garmsar Branch, Garmsar, Iran Romina Rajabi Faculty of Veterinary Medicine, Islamic Azad University, Science and Research Branch, Tehran, Iran Amirohssein Marashi Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran Mohammad Amin Dadboud Faculty of Veterinary Medicine, Razi University of Kermanshah, Kermanshah, Iran Hamidreza Majidiani Department of Basic Medical Sciences, Faculty of Medicine, Neyshabur University of Medical Sciences, Neyshabur, Iran Parham Rahmanian Faculty of Veterinary Medicine, Islamic Azad University, Science and Research Branch, Tehran, Iran 2024 11 13 2025 02 09 Background: A neglected zoonosis, cystic echinococcosis (CE) is the most common diseas in the developing nations worldwide. Vaccination is helpful in preventing the disease. Predicting main biochemical properties of the E. granulosus annexin (ANX) and its possible B-cell and human leukocyte antigen (HLA)-binding epitopes as a viable vaccine candidate was the goal of the current study. Methods: This study was done in Neyshabur University of Medical Sciences, Neyshabur, Iran. Predictions about transmembrane domain, subcellular localization, post-translational modification (PTM) sites, physico-chemical, antigenic, and allergenic profiles, secondary and 3D structure, tertiary model refinement, and validations were done using online servers. Results: The cytoplasmic 79.05 kDa protein was non-allergenic, hydrophilic (GRAVY: -0.490), stable (instability: 39.30), with improved thermotolerance (aliphatic: 80.07) and 122 post-translational modification sites. The secondary structure mostly included helices and extended strands. The 3D model was generated using Robetta server (confidence: 0.59) and was refined and validated subsequently. Shared B-cell epitopes were discovered using ElliPro, ABCpred and SVMTriP servers with antigenicity, allergenicity, and solubility screening. Moreover, multiple human and mouse MHC-binding epitopes were predicted and screened in E. granulosus ANX. Conclusion: This work offers a foundation for further investigation regarding designing an effective vaccination against CE. Further empirical research using examined protein alone or in conjunction with other antigens/epitopes is needed in the future. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/4429 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/4429/1444