Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 19 4 2024 11 24 440 447 EN Bruna Baccega Parasitology Laboratory, Department of Microbiology and Parasitology, Federal University of Pelotas, Pelotas, Brazil Juliana Monteli Fenalti Parasitology Laboratory, Department of Microbiology and Parasitology, Federal University of Pelotas, Pelotas, Brazil Alexia Brauner de Mello Parasitology Laboratory, Department of Microbiology and Parasitology, Federal University of Pelotas, Pelotas, Brazil Yan Wahast Islabão Parasitology Laboratory, Department of Microbiology and Parasitology, Federal University of Pelotas, Pelotas, Brazil Paloma Taborda Birmann Neurobiotechnology Research Group, Postgraduate Program in Biotechnology, Technological Development Center, UFPel, Brazil Alessandra Neis Postgraduate Program in Biotechnology, Technological Development Center, UFPel, Brazil Marjorie de Giacomti Parasitology Laboratory, Department of Microbiology and Parasitology, Federal University of Pelotas, Pelotas, Brazil Francielle Liz Monteiro Laboratory of Virology and Immunology, Faculty of Veterinary, UFPel, Brazil Silvia de Oliveira Hubner Laboratory of Virology and Immunology, Faculty of Veterinary, UFPel, Brazil Rodrigo de Almeida Vaucher Research Laboratory in Biochemistry and Molecular Biology of Microorganisms (LaPeBBioM), UFPel, Brazil Nara Amélia da Rosa Farias Parasitology Laboratory, Department of Microbiology and Parasitology, Federal University of Pelotas, Pelotas, Brazil Roghayeh Norouzi Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran Abolghasem Siyadatpanah Infectious Diseases Research Center, Gonabad University of Medical Sciences, Gonabad, Iran Camila Belmonte Oliveira Parasitology Laboratory, Department of Microbiology and Parasitology, Federal University of Pelotas, Pelotas, Brazil 2024 04 22 2024 10 15 Background: Avian trichomoniasis is an important disease that causes bird mortality, both wild and captive, around the world. This study evaluated the in vitro cytotoxicity, antioxidant, and antiparasitic activity of citral (3.7-Dimetil-2.6-octadienal) and geraniol (trans-3.7-Dimetil-2.6-octadien-1-ol) against Trichomonas gallinae trophozoites. Methods: In vitro assays were conducted at the Laboratory of Protozoology and Entomology (LAPEN) at the Federal University of Pelotas (UFPel), Brazil in 2019 using tests with 106 parasites and citral and geraniol at concentrations ranging from 10 to 100 μM and four controls: NC (culture medium and trophozoites), MTZ (trophozoites plus 100 μM of metronidazole), and TW (trophozoites plus vehicles used for solubilizing derivatives (0.01% Tween). Results: The citral (60 μM) and geraniol (50 μM) concentrations reduced the trophozoitesʼs viability by 100%. The molecular docking experiment demonstrated that citral and geraniol might inhibit a hydrogen enzyme for T. gallinae survival. Conclusion: The major compounds of lemongrass have potential antitrichomonal activity against T. gallinae in vitro. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/4250 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/4250/1390