Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 19 4 2024 11 24 418 427 Ana Cristina González-Morales Department of Invertebrates Zoology, Biological Sciences School, Autonomous University of Nuevo León, San Nicolás de los Garza, Nuevo León, México Zinnia Judith Molina-Garza Department of Invertebrates Zoology, Biological Sciences School, Autonomous University of Nuevo León, San Nicolás de los Garza, Nuevo León, México Ricardo Gomez-Flores Department of Immunology and Virology, Biological Sciences School, Autonomous University of Nuevo León, San Nicolás de los Garza, Nuevo León, México Juan José Zárate-Ramos Department of Parasitology, Agricultural Sciences Campus, Veterinary Medicine and Zootechnics School, Autonomous University of Nuevo León, San Nicolás de los Garza, Nuevo León, México Lucio Galaviz-Silva Department of Invertebrates Zoology, Biological Sciences School, Autonomous University of Nuevo León, San Nicolás de los Garza, Nuevo León, México 2024 01 29 2024 08 11 Background: We aimed to develop a sandwich ELISA, using polyclonal antibodies against excretory/secretory (E/S) antigens specific to coproantigens present in Toxocara canis-positive dogs. Methods: Antibodies were produced at Biological Sciences School, Autonomous University of Nuevo León, México in 2023 by immunization of rabbits with antigenic extracts from in vitro cultures of T. canis larvae. Assays were performed on 100 stool samples from pet dogs, measuring sensitivity, specificity, and cross-reactivity against other parasitic infections. Results: High values of sensitivity (100%), specificity (90.9%), and positive (93.47%) and negative (95.45%) predictive values were obtained, respectively. We obtained an E/S protein with a molecular weight of 70 kDa, which showed high sensitivity and specificity by ELISA, but it presented cross-reactivity against Ancylostoma caninum and Strongyloides stercoralis. Conclusion: Additional studies are necessary to increase the specificity percentage since this assay demonstrated significant potential as a useful and inexpensive diagnostic tool for immunodiagnostic in dog feces. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/4135 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/4135/1388