Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 19 1 2024 02 28 9 17 Selahattin Aydemir Department of Parasitology, Faculty of Medicine, Van Yüzüncü Yıl University, Van, Turkey Fethi Barlık Department of Parasitology, Faculty of Medicine, Van Yüzüncü Yıl University, Van, Turkey Abdurrahman Ekici Department of Parasitology, Faculty of Medicine, Van Yüzüncü Yıl University, Van, Turkey Dilara Hande Barlık Department of Molecular Biology and Genetics, Faculty of Science, Van Yüzüncü Yıl University, Van, Turkey Sevil Alkan Department of Infectious Disease and Clinical Microbiology, Faculty of Medicine, Çanakkale Onsekiz Mart Uni-versity, Çanakkale, Turkey Esra Gürbüz Infectious Diseases and Clinical Microbiology, SBU Van Training and Research Hospital, Van, Turkey Hasan Yilmaz Department of Parasitology, Faculty of Medicine, Van Yüzüncü Yıl University, Van, Turkey 2023 07 04 2024 02 28 Background: We aimed to determine the prevalence of Giardia intestinalis and Cryptosporidium spp. in patients who admitted hospital with diarrhea and to gain information about the transmission of these parasites in Ağrı, Türkiye. Methods: This study included 184 patients who applied to Ağrı-Diyadin State Hospital, Türkiye in 2022. The immunochromatographic card test was used for detection of the G. intestinalis and Cryptosporidium spp. Nested PCR-RFLP analysis of the COWP gene and sequence analysis of the gp60 gene were used to genotype and subtype Cryptosporidium spp., whereas Nested PCR and sequence analyses of β-giardin gene were used genotype G. intestinalis. Results: Of the 184 stool specimens examined, 12 (14.29%) and 7 (3.80%) were positive for G. intestinalis and Cryptosporidium spp., respectively. The Cryptosporidium species were identified as C. parvum belonging to the IId subtype family. The G. intestinalis were identified assemblages A. Conclusion: Assemblage A, which is associated with diarrhea, is responsible for giardiasis and C. parvum IId subtype, often found in sheep, goats and cattle, is responsible for cryptosporidiosis in Ağrı, Türkiye. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3993 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/3993/1336