Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 18 3 2023 09 22 382 389 Guo Yue School of Medicine, Huzhou University & Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou, Zhejiang, China Yu Liang Cao Emergency Department, South Taihu Hospital, Gangnan Road 1566, Huzhou, Zhejiang, China Dong Ying School of Medicine, Huzhou University & Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Prov-ince, Huzhou, Zhejiang, China Zhou Hongchang School of Medicine, Huzhou University & Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Prov-ince, Huzhou, Zhejiang, China Shang Ya Qian School of Medicine, Huzhou University & Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Prov-ince, Huzhou, Zhejiang, Chinaa Zhang De Long School of Medicine, Huzhou University & Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Prov-ince, Huzhou, Zhejiang, China Chen Gao Qiang School of Medicine, Huzhou University & Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Prov-ince, Huzhou, Zhejiang, China Jiang Bei School of Medicine, Huzhou University & Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Prov-ince, Huzhou, Zhejiang, China Wu Wan Ting School of Medicine, Huzhou University & Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Prov-ince, Huzhou, Zhejiang, China Lu Mingke School of Life Sciences, Xiamen University, Fujian, China 2023 02 08 2023 09 22 Background: The pathogen of angiostrongyliasis is the parasite Angiostrongylus cantonensis, and the transcriptome profiling of the male adult was unclear. We aimed to understand how the male adults adapt, so the expression profile of A. cantonensis adult males was analyzed. Methods: In order to improve the understanding of the transcriptome of adult males, RNA from three groups of male adult A. cantonensis was extracted and reverse transcribed to construct cDNA libraries. After sequencing, annotation of unigenes and transcripts was performed by querying the NR (Non-Redundant Protein Sequence Database), GO (Gene Ontology) and COG/ KOG (Clusters of Orthologous Groups of proteins/euKaryotic Ortholog Groups) databases. Results: For each group of adults, 43,260,894 raw reads and 43,200,341 clean reads were obtained. After successful assembly, 87,649 unigenes and 146,895 transcripts were obtained. Annotation of the unigenes and transcripts was identical and male adults expressed a series of genes encoding proteins specific to the male gender at the adult stage, such as proteins involved in energy metabolism, energy synthesis and transport. Expression of the ribosome pathway suggests a relationship with the physical activities during the adult male stage. Conclusion: The transcriptome analysis is a good reference to understand further the expression profile of male adult A. cantonensis. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3872 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/3872/1311