Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 9 3 2014 09 15 394 401 EN Li-Jun Jia Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Yanbian University, Yanji, Jilin, China. Shou-Fa Zhang Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Yanbian University, Yanji, Jilin, China. Ming-Ming Liu Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Yanbian University, Yanji, Jilin, China. Nian-Chao Qian Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Yanbian University, Yanji, Jilin, China. Huan-Ping Guo Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Yanbian University, Yanji, Jilin, China. 2015 10 14 Background: The aim of the study was to provide a point of reference to study the Neospora caninum infections in China. Methods: Genome DNA was extracted from the brains of aborted fetuses and specific PCR was performed with N. caninum Nc5-targeted specific primers. Fetal bovine brain tissues were homogenized and continuously cultured in Vero cells with double antibodies. The medium was replaced at 2-d intervals and the state of cells was observed. Results: A 608 bp Nc5 gene band was detected by PCR amplification. After sequencing,the sequence of the sample shared 99.5% homology with GenBank(AF061249). Brain homogenates were continuously cultured in Vero cells for 34 d and N. caninum was found. The results of IFAT and Nc5 gene-based PCR detection were N. caninum-positive, and the parasite was tentatively named N. caninum China Yanbian strain. BABL/c mice were inoculated with the separated parasites and showed clinical symptoms of ataxia and limb paralysis after 12 d. Only 3 mice survived.The blood of dying mice and the hearts, livers, spleens, lungs, kidneys, andbrains of dead mice were collected aseptically. The Nc5 gene-based PCR showed that N. caninum may exist in brains, livers, and spleen. Based on immunohistochemical observations, we showed that N. caninum tachyzoites existed in the brains and livers. Conclusion: We have successfully isolated bovine-specific N. caninum strain from brain tissues of aborted cattle in the China Yanbian region. This isolated strain has a strong infectious ability towards BABL/c mice. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/387 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/387/357