Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 17 4 2022 12 04 506 516 EN Asli Zorba Yildiz 1. Department of Bioengineering, Faculty of Chemical and Metallurgical Engineering, Yildiz Technical University, Istanbul, Turkey 2. Department of Medical Services and Techniques, School of Vocational of Healty, Istinye University, Istanbul, Turkey Gulnaz Yildirim Koken 1. Department of Bioengineering, Faculty of Chemical and Metallurgical Engineering, Yildiz Technical University, Istanbul, Turkey 2. Liv Hospital Regenerative Medicine and Stem Cell Production Center, Istanbul, Turkey Emrah Abamor Department of Bioengineering, Faculty of Chemical and Metallurgical Engineering, Yildiz Technical University, Istanbul, Turkey Melahat Bagirova 1. Department of Bioengineering, Faculty of Chemical and Metallurgical Engineering, Yildiz Technical University, Istanbul, Turkey 2. Institute of the V. Akhundov National Scientific Research Medical Prophylactic, Baku, Azerbaijan Republic Biomedical Ozlem Tosyali Department of Biomedical Engineering, Faculty of Engineering Architecture, Beykent University, Istanbul, Turkey Tanil Kocagoz Department of Basic Sciences, Faculty of Medicine, Acibadem Mehmet Ali Aydinlar University, Istanbul, Turkey Adil Allahverdiyev Department of Bioengineering, Faculty of Chemical and Metallurgical Engineering, Yildiz Technical University, Istanbul, Turkey 2. Institute of the V. Akhundov National Scientific Research Medical Prophylactic, Baku, Azerbaijan Republic 2021 12 04 2022 07 31 Background: Leishmaniasis is a zoonotic disease, which is one of the serious public health problems in the world. Nowadays, antibody production using hybridoma technology may be a correct approach in terms of sensitivity in the diagnosis of diseases such as leishmaniasis. The aim of this study was investigation of the effectiveness of different adjuvants on polyclonal antibody production against L. tropica based on hybridoma technique. Methods: Accordingly, Freund's adjuvant (1956, M. tuberculosis), as a classic adjuvant in studies, was used comparatively with the non-toxic polymeric based Polyoxidonium adjuvant. All animal immunization procedures were conducted at Bezm-i Alem University Experimental Animal Research Center. The adjuvant response was tested both in the serum sample and in the antibodies produced by the hybridomas. The antibody titers were determined with ELISA. Results: Freund’s and Polyoxidonium (PO) group blood titer’s increased approximately 5.5 fold compared to control after the 6th and 8th immunization. Hybridomas produced from mice immunized with PO adjuvant induced only antigen-specific antibody response and did not develop an immune response against the adjuvant. Conclusion: Adjuvant selection is very important in terms of the specificity of antibody responses of cells produced in hybridoma technology. Therefore, PO is recommended as a new adjuvant system in this study. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3514 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/3514/1250