Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 10 1 2015 03 15 110 115 EN Shirzad Gholami Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran. Farhang Babamahmoodi Antimicrobial Resistance Research Center, Mazandaran University of Medical Sciences, Sari, Iran. Rohallah Abedian Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran. Mehdi Sharif Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran. Abbas Shahbazi Research Center of Infectious and Tropical Diseases, Tabriz University of Medical Sciences, Tabriz, Iran. Abdolsattar Pagheh Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran. Mahdi Fakhar Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran. 2015 10 14   Background: Infection with Trichostrongylus spp. is common among human and herbivorous in most parts of Iran, especially in southern and northern ar-eas. The aim of present study was to identify Trichostrongylus spp. among hu-man population using excreted egg specimens, by the molecular method, in Mazandaran Province, northern Iran. Methods:Overall, 33 positive fecal specimens were randomly sampled and examined. PCR amplification of ITS2-rDNA region was performed on the isolated egg and then a restriction fragment length polymorphism (RFLP) profile was considered to discriminate of Trichostrongylus spp. Results:A total of 33 positive fecal specimens, 29(78.9%), 4(12.1%) were found T. colubriformis and T. axei respectively. Our data appear the molecular evidence of both human T. colubriformis and T. axei infections in North of Iran. Conclusion:T. colubriformis was the probable most common zoonotic species caus-ing human trichostrongylosis infection in the area https://ijpa.tums.ac.ir/index.php/ijpa/article/view/345 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/345/430