Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 10 2 2015 06 15 206 212 EN Mohammad Reza Lashkarizadeh Dept. of Surgery and the Research Center for Tropical and Infectious diseases, Kerman University of Medical Sciences, Kerman, Iran. Keivan Asgaripour Dept. of Surgery and the Research Center for Tropical and Infectious diseases, Kerman University of Medical Sciences, Kerman, Iran. Ebrahim Saedi Dezaki Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran. Majid Fasihi Harandi Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran. 2015 10 14   Background:Haemonchosis has a negative effect on the farming industry throughout the world, especially in the tropic and sub-tropic countries. The present study was carried out to differentiate Haemonchus species from its main hosts in Iran, including sheep, goat and camel.  Methods:The identification took place based on the morphometrics of the spic-ules and molecular characters. Two hundred seventy adult male nematodes were collected from the abomasums of different ruminants (90 samples from each ani-mal) at the slaughterhouses from different localities in Iran. Samples were morpho-logically identified according to the spicules’ morphometric measurements. In the section on molecular study, 10 samples of each Haemonchus isolates were genetically examined. A simple PCR-restriction fragment length polymorphism (PCR-RFLP) assay of the second internal transcribed spacer of ribosomal DNA (ITS2-rDNA) were described to confirm the PCR results. Results:PCR-RFLP profile obtained from the restriction enzyme HPa1 in H. con-tortus and H. longistipes indicated 1 (278 bp) and 2 (113 and 135 bp) different frag-ments, respectively. The morphological parameters clearly distinguish H. contortus from H. longistipes. Moreover, regarding the ITS2-rDNA, sequences of 295 bp and 314 bp were obtained from H. contortus and H. longistipes, respectively. Conclusion:High similarity of cloned PvMSP-142 kDa gene in comparison to reference sequence and other sequences could be beneficial as a remarkable mo-lecular marker for serological diagnostic. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/331 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/331/470