Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 10 2 2015 06 15 245 249 EN Gholamreza Mowlavi Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran AND Dept. of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Mahsasadat Makki Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran. Zahra Heidari Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran. Mostafa Rezaeian Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran AND Dept. of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Mehdi Mohebali Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran AND Dept. of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Adauto Araujo Escola Nacional de Saúde Pública, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil (Brazilian School of Public Health, Oswaldo Cruz Foundation). Nicole Boenke Ruhr Universität Bochum, Institut für Archäologische Wissenschaften, Am Bergbaumuseum 31, D-44791 Bochum, Germany. Abolfazl Aali Archaeological Museum of Zanjan, Emaarate Zolfaghari, Taleghanist., Zanjan, Iran. Thomas Stollner Ruhr Universität Bochum, Institut für Archäologische Wissenschaften, Am Bergbaumuseum 31, D-44791 Bochum, Germany. Iraj Mobedi Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran AND Dept. of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. 2015 10 14   Present paper is the second publication introducing the paleoparasitological findings from animal coprolites obtained from archeological site of Chehrabad salt mine in northwest-ern Iran. The current archeological site is located in northwest of Iran , dated to the Sas-sanian Era (4th/5th century CE).In the summer 2012 the carnivore coprolite was obtained within the layers in the mine and were thoroughly analyzed for parasites using TSP re-hydration technique. Eggs of Macracanthorhynchus hirudinaceuswere successfully retrieved from the examined coprolite and were confidently identified based on reliable references. Identifying of M.hirudinaceuseggs in paleofeces with clear appearance as demonstrated herein, is much due to appropriate preservation condition has been existed in the salt mine .The present finding could be regarded as the oldest acanthocephalan infection in Iran. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/327 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/327/476