Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 15 3 2020 09 12 418 424 Negar BAHRAM Student Research Committee, Arak University of Medical Sciences, Arak, Iran Mitra SHARBATKHORI Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran AND Department of Parasitology & Mycology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran Farideh TOHIDI Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran Reza GHASEMIKHAH Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran AND Department of Parasitology & Mycology, School of Medicine, Arak University of Medical Sciences, Arak, Iran 2020 09 12 Background: Fascioliasis is a neglected zoonotic disease, caused by Fasciola species in human and livestock. We aimed to detect the seroprevalence of human fascioliasis Gorgan City, Golestan Province, northern Iran using ELISA method in 2017. Methods: Overall, 612 serum samples were analyzed. A relevant questionnaire for demographic data was obtained for all cases. An indirect ELISA test was used to detect IgG antibodies against Fasciola in the sera. The data analysis was performed employing SPSS program version 21. Results: Eleven cases (1.79%) were seropositive for fascioliasis. The seroprevalence of fascioliasis was 1.9% and 1.1% among males and females, respectively. There was no statistically significant association between the fascioliasis and analyzed variables such as sex, age, residence, job, education, etc. Conclusion: This study was conducted only on the people referring to the Reference Laboratory of Gorgan. It cannot be distributed to the whole city. Thus, due to importance of the disease, finding the seroprevalence of fascioliasis in a comprehensive survey in Golestan Province should be accounted in further studies. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3069 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/3069/1072