Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 14 2 2019 06 19 240 249 EN Enas Fakhry ABDELHAMED Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Sharkia Governorate, Egypt Eman Magdy FAWZY Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Sharkia Governorate, Egypt Said Mahmoud AHMED Department of Zoology, Faculty of Science, Zagazig University, Sharkia Governorate, Egypt Rabab Sayed ZALAT Department of Medical Parasitology, Theodor Bilharz Research Institute, Giza, Egypt Hayam Elsaid RASHED Department of Pathology, Faculty of Medicine, Zagazig University, Sharkia Governorate, Egypt 2018 03 21 2018 09 03 Background Cryptosporidium parvum  is a dangerous intestinal pathogen due to its  devastating  effect in immunocompromised individuals. Considering low efficacy, high toxicity in addition to the development of resistance for the drugs used, this study aimed to find a new alternative treatment having the advantage of lower doses and minimal toxicity. As complementary for our previous research, we used a novel combination between artesunate loaded polymeric nanofiber and nanozoxide that had not been tried yet. Methods Our experiment was performed on 60 immunocompromised mice that divided into six groups of 10 mice each: G I: infected control, G II: infected and treated with nanozoxide, G III: infected and treated with combination of artesunate, G IV: infected and treated with combination of nanozoxide and artesunate loaded nanofiber, G V: infected and treated with artesunate-loaded nanofiber, Group VI: uninfected control. Group IV had the highest oocyst reduction rate. Results The novel combination between artesunate loaded polymeric nanofiber and nanozoxide  has a harmonizing effect in reducing oocyst shedding, increasing total antioxidants activity, decreasing oxidative stress in tissues in addition to the marked improvement of histopathological features. Conclusions This combination has a promising therapeutic effect against cryptosporidiosis particularly in immunocompromised individuals considering minor toxicity. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2065 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/2065/940