Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 6 3 2011 09 15 74 81 EN MJ Gharavi Department of Medical Parasitology, Tehran University of Medical Sciences, Tehran, Iran AND Research Institute for Islamic & Complementary Medicine, Tehran University of Medical Sciences, Tehran, Iran M Nobakht Department of Histology and Neuroscience, School of Medicine, Tehran University of Medical Sciences,Tehran, Iran AND Research institute for Islamic & Complementary Medicine, Tehran University of Medical Sciences Tehran, Iran SH Khademvatan Department of Parasitology, Jundishapur University of Medical Sciences, Ahvaz, Iran E Bandani Fculty of Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran M Bakhshayesh Cellular and Molecular Research Center, Tehran University of Medical Sciences, Tehran, Iran M Roozbehani Fculty of Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran 2015 10 03 Background: The study was aimed to show the effect of molecular mechanism of Aqueous Garlic Extract (AGE) on expression of IFNγ and iNOS genes in Leishmania major. Methods: Leishmania major promastigotes (MRHO/IR/75/ER) were added to the in-vitro cultured J774 cell line, the cells were incubated for 72 hours. Various concentrations of garlic extract (9.25, 18.5, 37, 74, 148 mg/ml) were added to the infected cells. MTT assay was applied for cellular proliferation. After 72 hours of incubation, supernatants were collected and total RNA was extracted from the infected cells. The express of IFNγ and iNOS genes were studied by RT-PCR method. Results: The colorimetric MTT assay after 3 days of incubation showed cytotoxic effect of garlic extract with an IC50 of 37 mg/ml. In addition, IFNγ and iNOS genes expression by RT-PCR indicated that garlic extract lead to over expression of these genes in J774  cell line infected with L. major. Conclusion: Garlic extract exerts cytotoxic effect on infected J774 cell line. In addition, the hypothesis that garlic can improve cellular immunity with raising the expression of IFNγ and of iNOS genes con­firmed. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/191 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/191/190