Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 6 2 2011 06 15 69 73 EN M Mohebali Department of Medical Parasitology and Mycology , School of Public Health, Tehran University of Medical Sciences, Tehran , Iran AND Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran A Malmasi Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran H Hajjaran Department of Medical Parasitology and Mycology , School of Public Health, Tehran University of Medical Sciences, Tehran , Iran AND Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran S Jamshidi Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran B Akhoundi Department of Medical Parasitology and Mycology , School of Public Health, Tehran University of Medical Sciences, Tehran , Iran AND Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran M Rezaei Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran S Janitabar Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran H Zarei Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran S Charehdar Department of Medical Parasitology and Mycology , School of Public Health, Tehran University of Medical Sciences, Tehran , Iran AND Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran 2015 10 03 A 5-month old puppy with muco-cutaneous lesions in the chin, around lips and eyes was exam­ined physically and microscopically for leishmaniasis. Muco-cutaneous lesions containing a large num­ber of amastigotes of Leishmania spp. were observed. Amastigotes were also detected in liver and spleen of the puppy. The animal was positive with Dipstick rK39 kit and high level of anti-Leishmania antibodies was detected by direct agglutination test (DAT). DNA, Using PCR-RFLP technique extracted from cultured Leishmania promastigotes and L. tropica was identified. This is the first report of concurrent mucosal and visceral involvement of L. tropica in a puppy from Iran. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/182 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/182/181