Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 12 3 2017 09 17 441 445 Shabnam JEIBOUEI Dept. of Parasitology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mojgan BANDEHPOUR Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran AND Dept. of Biotechnology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Bahram KAZEMI Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran AND Dept. of Biotechnology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Ali HAGHIGHI Dept. of Parasitology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2017 09 17 2017 09 17 Background: Leishmaniasis is a neglected disease affecting millions of people worldwide. The treatment of the disease is hampered due to high cost, toxicity and the crisis of drug resistance. Polytope approaches of genetic immunization could be a strategy for prevention of infectious diseases. Furthermore, the identification of Leishmania genome sequence and the application of bioinformatics assist us to devise an effective vaccine’s candidate. Methods: A linear sequence from predicted epitopes of GP63, LACK and CPC antigens was designed and was optimized using online available algorithms. The synthesized sequence (LAKJB93) was ligated to pEGFP-N1 plasmid. Results: The 264bp sequence was cloned at N terminal of GFP into pEGFP_N1 expression vector and transfect into CHO cell line. Construct was efficient expressed in CHO cells. Conclusion: The protein of LAKJB93 cosnstruct was expressed in CHO cells successfully. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/1776 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/1776/767