Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 14 1 2019 03 11 120 126 EN Salma TEIMOORI Center of Excellence for Therapeutic Proteins and Antibody Engineering, Department of Parasitology, Faculty of Medicine, Siriraj Hospital, Bangkok 10700, Thailand AND WHO Collaborating Center for Research and Control of Opisthorchiasis, Tropical Disease Research Laboratory, Department of Experimental Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand Gholamreza MOWLAVI Department of Parasitology and Mycology, Tehran University of Medical Sciences, Tehran, Iran Yuji ARIMATSU WHO Collaborating Center for Research and Control of Opisthorchiasis, Tropical Disease Research Laboratory, Department of Experimental Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand Banchob SRIPA WHO Collaborating Center for Research and Control of Opisthorchiasis, Tropical Disease Research Laboratory, Department of Experimental Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand Iraj MOBEDI Department of Parasitology and Mycology, Tehran University of Medical Sciences, Tehran, Iran Meysam SHARIFDINI Department of Medical Microbiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran Jafar MASSOUD Department of Parasitology and Mycology, Tehran University of Medical Sciences, Tehran, Iran Saied Reza NADDAF Department of Parasitology, Research Center for Emerging and Reemerging Diseases, Pasteur Institute of Iran, Tehran, Iran 2017 07 28 2019 03 11 Background: We detected eight trematodes in the small intestine of a road-killed jackal (Canis aureus) from Hamidiyeh District near the city of Ahvaz, Khuzestan Province in 2010. Methods: Three worms were stained with carmine acid, mounted in Canada balsam on glass slides and examined under a light microscope at 1000X magnification. PCR and sequencing of a partial ITS2 sequence were used to approve the diagnosis. Results: The flukes measured ≈1 mm in length with an elongated ovoid shape resembling the members of heterophyid, and only one testis, characteristics of the genus Haplorchis. Sequencing of a 481-bp fragment of the ITS2 locus from the worms revealed 97%-98% identity with the similar sequences of the H. taichui flukes previously identified in the fish, cat, and humans from Thailand, China, and Vietnam. Conclusion: Further studies with the application of reliable molecular tools to diagnose trematode infections in wildlife and humans can bring more insight into the epidemiology of fish-borne flukes including H. taichui in this area. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/1705 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/1705/920