Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 6 1 2011 03 15 81 85 EN M Hosseininejad Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran AND Research Institute of Animal Embryo Technology, University of Shahrekord, Iran AND Research Institute of Zoonotic Diseases, University of Shahrekord, Shahrekord, Iran A Malmasi Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran F Hosseini Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran M Selk-Ghaffari Faculty of Veterinary Medicine, Islamic Azad University- Karadj Branch, Karadj, Iran N Khorrami Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran M Mohebali School of Public Health, Tehran University of Medical Sciences, Tehran, Iran S Shojaee School of Public Health, Tehran University of Medical Sciences, Tehran, Iran A Mirani Research Institute of Zoonotic Diseases, University of Shahrekord, Shahrekord, Iran M Azizzadeh Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran P Mirshokraei Research Institute of Animal Embryo Technology, University of Shahrekord, Iran A Aliari Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran 2015 10 03 Background: Toxoplasma gondii infects a wide range of animals; felines are definitive hosts and other animals including the dogs are intermediate hosts. The aim of this study was to determine the seroprevalence of T. gondii infection in dogs in Tehran, capital of Iran and to investigate possi­ble associated risk factors. Methods: Three hundreds ninety six serum samples were collected during 2007-8 from the dogs. Col­lected samples were tested using an indirect fluorescent antibody test (IFAT) in dilutions of 1:16 and more. All procedures were carried out in Shahrekord University, Iran. All the data were analyzed using SPSS software, qui square test with confidence interval of 0.95. Results: From evaluated samples, 89 (22.47%) were positive in titers of at least 1:16. further evalua­tions in other dilutions showed positive results in dilutions of maximum 1:16 , 1:32, 1:64, 1:128 and 1:256 in 38, 29, 15, 2 and 5 dogs respectively. Investigation of the role of risk factors showed no sex predisposition while infection rate was significantly higher in dogs older than one year old. Living places were of significant importance; infection rate was significantly higher in stray or guard dogs in compare with household dogs (P<0.05). Conclusion: Relatively high seroprevalence of T. gondii infection in dogs in Tehran shows high environmental contamination. It is recommended that the dogs with suspected clinical signs be tested for T. gondii infection. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/169 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/169/168