Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 6 1 2011 03 15 28 33 EN S Jafar pour Azami Dept of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran H Keshavarz Dept of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran M Rezaian Dept of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran M Mohebali Dept of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran S Shojaee Dept of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2015 10 03 Background: Toxoplasmosis is a worldwide endemic disease. In congenitally infected infants and AIDS patients, toxoplasmosis causes high rates of morbidity and mortality. In these cases antibody detection is difficult; so detection of parasite or its components could be useful tool for early detection and following treatment of the infection. Methods: Sixty-three BALB/c mice were injected intra-peritoneal with 5×103 tachyzoites of Toxoplasma gondii RH strain, nine mice were sacrificed daily for 7 days. Fourteen mice were in­jected with phosphate buffer saline as control group. Dot-ELISA was performed for detection of T.gondii antigen in mice sera and capture - ELISA was done as golden standard assay too. Results : Toxoplasma gondii antigen was detected from day 2 in mice sera ; 22% of mice sera on day 2, 33% on day 3,77% on day 4 and 100% on day 5 till their death on day 7 had shown antigene­mia by dot - ELISA, no positive result was detected in control mice by dot- ELISA. Conclusion: Dot-ELISA is a sensitive method for diagnosis of T. gondii infection in the animal model; also, this technique is more rapid and easy to perform method in comparison with cap­ture-ELISA. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/162 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/162/161