Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 13 3 2018 09 24 Allelic Variations of Plasmodium vivax Apical Membrane Antigen-1 (Pv AMA-1) in Malarious Areas of Southeastern Iran Using PCR-RFLP Technique 473 479 EN Afsaneh MOTEVALLI HAGHI Dept. of Medical Parasitology and Mycology, School of Public Health, International Campus, Tehran University of Medical Sciences, Tehran, Iran Sepide MORADI Dept. of Medical Parasitology and Mycology, School of Public Health, International Campus, Tehran University of Medical Sciences, Tehran, Iran Mehdi NATEGHPOUR Dept. of Medical Parasitology and Mycology, School of Public Health, International Campus, Tehran University of Medical Sciences, Tehran, Iran AND Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran Gholamhossein EDRISSIAN Dept. of Medical Parasitology and Mycology, School of Public Health, International Campus, Tehran University of Medical Sciences, Tehran, Iran 2016 11 07 2017 07 18 Background: Although Plasmodium vivax is usually known as benign malaria, some variations of the parasite can result in acute and sever infection. In this study we tried to determine some genetic variations in PvAMA-1 antigen among the samples were collected form southeastern Iran. Methods: About two ml blood samples were collected into EDTA pre-dosed tubes from 30 P. vivax–infected patients individually between 2011 and 2013. A Giemsa stained thick and thin blood film was prepared from each of the patients. A PCR-RFLP technique was employed using EcoR-1, Pvu-II and Hind3 restriction enzymes to determine the allelic variations of the antigen. Results: A 1300bp gene corresponding to PvAMA-1 was selected for the amplification process. Among the total cases identified in this study 90% showed similar bounds when exposed to the restriction enzymes. Nine isolates (accession numbers: KF435081-KF435083 and JF682785-JF682790) were identified and registered in Gene bank. Identity among isolates was more than 96% in nucleotide level. Dendrogram clarified a close relationship among the clusters in spite of geographical distribution of the parasite. Conclusion: This study increased our data about prevalence and variation of PvAMA-1 alleles amongst P. vivax isolates in southeastern parts of Iran where besides native population bears considerable Afghan and Pakistani immigrants. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/1285 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/1285/862