Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 5 1 2010 03 15 41 46 EN MB Rokni Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran M Aryaeipour Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran S Koosha Parasitology and Mycology Division, Laboratory Sciences Department, Paramedical School, Shaheed Beheshti University M.C., Tehran, Iran MT Rahimi Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2015 10 03 Background: Considering that ELISA method presently is the test of choice for diagnosis of fasciolo­sis, the present study was undertaken to evaluate the maximum validity of coated plates at dif­ferent temperatures and different times during one year of evaluation. Methods: Serum samples of patients infected with fasciolosis (n=10), hydatidosis (n=5), toxocaria­sis (n=5), and negative control sera (n=5) were examined. Two series of plates were consid­ered. The first series were coated with Fasciola homogenate Ag 12 ug/ml, and after some steps were blocked with gelatin and preserved at different temperatures as -80 °C , -20 °C, -4 °C and +4°C. The 2nd series were treated under the same criteria but were not blocked with gelatin. Each series were examined by ELISA test from 1st month to 12th month. Sera with 1:125 dilution, and peroxidase-conjugated goat anti-human IgG diluted 1:10000 were considered optimum. Results: To ease reporting the results and due to many similarities only results related to 1st, 6th and 12th months were analyzed and sensitivity, specificity plus cut-off were determined for each series separately. Conclusion: Preserving the coated plates, while unblocked at -80°C for 6-8 months is pertinent and functional and in that case, we can be sure the best out put would be applicable. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/125 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/125/124