Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 5 1 2010 03 15 20 24 EN A Farahnak Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran T Golmohamadi Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Iran MB Molaei Rad Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2015 10 03 Background: Fascioliasis is a chronic hepatic disease and may be resulted from mechani­cal/molecular parasite adhesion to host liver tissue. The aim of this study was to detect surface car­bohydrate and lectin, carbohydrate-binding protein isolation that might be responsible of this molecular binding. Methods: The present experimental work was conducted in the Department of Medical Parasitol­ogy and Mycology, School of Public Health, Tehran University of Medical Sciences, Te­hran, Iran.  Fasciola hepatica parasites were collected from abattoir (Saman, Tehran, Iran) and surface mannose-carbohydrate was detected by fluorescein isothiocyanate (FITC) conju­gated lectin (Lentil). Lectin of tegumental tissue from F. hepatica was isolated by affinity chroma­tography and detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Results: Mannose carbohydrate was observed on the surface of tegumental tissue from para­site under fluorescence microscope. Carbohydrate-binding protein or lectin with MW of 50 kDa also was isolated from homogenized tegument of helminth. Conclusion: These results are important for understanding of molecular pathogenesis of F. hepat­ica at the chronic phase of fascioliasis https://ijpa.tums.ac.ir/index.php/ijpa/article/view/122 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/122/121