Tehran University of Medical Sciences Iranian Journal of Parasitology 1735-7020 4 3 2009 09 15 1 9 SH Hejazi N Zia- Jahromi M Bandehpour G Eslami R Salehi A Khamesipour B Kazemi 2015 10 03 Background: Leishmania is an obligatory intracellular protozoan parasite, which infects human be­ings when infected sand fly vector takes a blood meal.  Most efforts are towards designing an effective vaccine to prevent leishmaniasis. In this way, development of candidate antigen for vaccine has spe­cial im­portant. In this study, we cloned mannose-1-phosphate guanyltransferase gene of Iranian L .major in pET32a expression vector. Methods: Primers based on L. major mannose-1-phosphate guanyltransferase sequence gene was de­signed and synthesized. DNA of Leishmania promastigotes was extracted and PCR reaction was done. PCR product was cloned into pTZ57R and sub cloned into pET32a expression vector. Results: Recombinant plasmid containing 1140 bp as L. major mannose-1-phosphate guanyltrans­ferase gene was extracted and confirmed by restriction analysis. PCR product was sequenced and de­posited to GenBank. There were some differences in amino acid sequences between Iranian L. major mannose-1-phosphate guanyltransferase and others previously accepted in GenBank Conclusion: We amplified and cloned Iranian L. major mannose-1-phosphate guanyltransferase suc­cessfully. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/102 https://ijpa.tums.ac.ir/index.php/ijpa/article/download/102/101