Iranian Journal of Parasitology 2016. 11(1):126-130.

Molecular Evaluation of a Case of Visceral Leishmaniasis Due to Leishmania tropica in southwestern Iran
Bahador SARKARI, Niloofar BAVARSAD AHMADPOUR, Abdolali MOSHFE, Homa HAJJARAN

Abstract


We describe a case of visceral leishmaniasis (VL) due to Leishmania tropica in a 50-year-old Iranian man lived in a VL-endemic area in southwest of Iran. The patient presented with a 3-month history of fever and splenomegaly. Clinical signs and serological findings were suggestive of VL. Spleen biopsy was taken from the patient and intracellular forms of Leishmania amastigotes was seen in Giemsa stained smears. The patient was treated with pentavalent antimonial compound with complete resolution of his systemic signs and symptoms. DNA was extracted from the microscopic slides of the spleen biopsy and the nagt (N-Acetylglucosamine-1-Phosphate Transferase) gene of Leishmania was PCR-amplified. Sequence analysis of the PCR product demonstrated that the case has 99% identity with those of available sequences of L. tropica. Intra-species variation within isolate was 0-0.1%; whereas, inter-species differences of the isolate with those of L. major and L. infantum was significantly higher.


Keywords


Visceral Leishmaniasis; Leishmania tropica; southwestern Iran

Full Text:

PDF

References


Antinori S, Schifanella L, Corbellino M. Leishmaniasis: new insights from an old and neglected disease. Eur J Clin Microbiol Infect Dis. 2012; 31(2):109-18.

Mohebali M. Visceral leishmaniasis in Iran: Review of the epidemiological and clinical features. Iran J Parasitol. 2013; 8(3):348-58.

Sarkari B, Hatam G, Ghatee M. Epidemiological features of visceral leishmaniasis in Fars province, southern Iran. Iran J Public Health. 2012; 41(4):94-9.

Sarkari B, Pedram N, Mohebali M, Moshfe AA, Zargar MA, et al. Seroepidemiological study of visceral leishmaniasis in Booyerahmad district, south-west Islamic Republic of Iran. East Mediterr Health J. 2010; 16(11):1133-6.

Sarkari B, Bavarsad N, Motazedian MH, Mir jalali H, Akhoundi M, et al. Inter and intra-specific variations of Leishmania strains isolated from cutaneous and visceral leishmaniasis patients in Fars province, south of Iran. Iran J Med Sci. 2016, 41 (in press).

Hajjaran H, Mohebali M, Mamishi S, Vasigheh F, Oshaghi MA, et al. Molecular identification and polymorphism determination of cutaneous and visceral leishmaniasis agents isolated from human and animal hosts in Iran. Biomed Res Int. 2013; 2013:789326.

Mohebali M, Hajjaran H, Hamzavi Y, Mobedi I, Arshi S, et al. Epidemiological aspects of canine visceral leishmaniosis in the Islamic Republic of Iran. Vet Parasitol. 2005; 129(3-4):243-51.

Mohebali M, Malmasi A, Hajjaran H, Jamshidi S, Akhoundi B, et al. Disseminated Leishmaniasis caused by Leishmania tropica in a puppy from Karaj, central Iran. Iran J Parasitol. 2011; 6(2):69-73.

Alborzi A, Rasouli M, Shamsizadeh A. Leishmania tropica-isolated patient with visceral leishmaniasis in southern Iran. Am J Trop Med Hyg. 2006; 74(2):306-7.

Karamian M, Motazedian MH, Mehrabani D, Gholami K. Leishmania major infection in a patient with visceral leishmaniasis: treatment with Amphotericin B. Parasitol Res. 2007; 101(5):1431-4.

Hyams KC, Hanson K, Wignall FS, Escamilla J, Oldfield EC. The impact of infectious diseases on the health of U.S. troops deployed to the Persian Gulf during operations Desert Shield and Desert Storm. Clin Infect Dis. 1995; 20(6):1497-504.

Alborzi A, Pouladfar GR, Fakhar M, Motazedian MH, Hatam GR, et al. Isolation of Leishmania tropica from a patient with visceral leishmaniasis and disseminated cutaneous leishmaniasis, southern Iran. Am J Trop Med Hyg. 2008; 79(3):435-7.

Jafari S, Hajiabdolbaghi M, Mohebali M, Hajjaran H, Hashemian H. Disseminated leishmaniasis caused by Leishmania tropica in HIV-positive patients in the Islamic Republic of Iran. East Mediterr Health J. 2010; 16(3):340-3.

Hajjaran H, Mohebali M, Zarei Z. Edrissian GhH. Leishmania tropica: Another etiological agent of canine visceral leishmaniasis in Iran. Iran J Public Health. 2007; 36:85-88.

Moshfe A, Mohebali M, Afshoun E, Mousavizadeh A, Zarei Z, et al. Canine visceral leishmaniasis in Boyer Ahmad district, Kohgiluyeh & Boyer Ahmad province, southwest of Iran. Iran J Parasitol. 2012; 7(4):75-81.

Hatam GR, Adnani SJ, Asgari Q, Fallah E, Motazedian MH, et al. First report of natural infection in cats with Leishmania infantum in Iran. Vector Borne Zoonotic Dis. 2010; 10(3):313-6.

Sarkari B, Hatam GR, Adnani SJ, Asgari Q. Seroprevalence of feline leishmaniasis in areas of Iran where Leishmania infantum is endemic. Ann Trop Med Parasitol. 2009; 103(3):275-7.

Krayter L, Bumb RA, Azmi K, Wuttke J, Malik MD, et al. Multilocus microsatellite typing reveals a genetic relationship but, also, genetic differences between Indian strains of Leishmania tropica causing cutaneous leishmaniasis and those causing visceral leishmaniasis. Parasit Vectors. 2014; 25(7):123.


Refbacks

  • There are currently no refbacks.


Creative Commons Attribution-NonCommercial 3.0

This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.