Ferredoxin Gene Mutation in Iranian Trichomonas vaginalis Isolates.

  • Soudabeh Heidari Parasitology and Mycology Dept. Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Mojgan Bandehpour Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran AND Biotechnology Dept. Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Seyyed-Javad Seyyed-Tabaei Parasitology and Mycology Dept. Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Zarintaj Valadkhani Parasitology Dept. Pasteur Institute of Iran, Tehran, Iran.
  • Ali Haghighi Parasitology and Mycology Dept. Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Alireza Abadi Social Medicine Dept. Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Bahram Kazemi Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran AND Biotechnology Dept. Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Keywords: Ferredoxin gene, Mutation, Trichomonas vaginalis

Abstract

 Background: Trichomonas vaginalis causes trichomoniasis and metronidazole is its chosen drug for treatment. Ferredoxin has role in electron transport and carbohydrate metabolism and the conversion of an inactive form of metronidazole (CO) to its active form (CPR). Ferredoxin gene mutations reduce gene expression and increase its resistance to metronidazole. In this study, the frequency of ferredoxin gene mutations in clinical isolates of T.vaginalis in Tehran has been studied. Methods: Forty six clinical T. vaginalis isolates of vaginal secretions and urine sediment were collected from Tehran Province since 2011 till 2012. DNA was extracted and ferredoxin gene was amplified by PCR technique. The ferredoxin gene PCR products were sequenced to determine gene mutations. Results: In four isolates (8.69%) point mutation at nucleotide position -239 (the translation start codon) of the ferredoxin gene were detected in which adenosine were converted to thymine. Conclusion: Mutation at nucleotide -239 ferredoxin gene reduces translational regulatory protein’s binding affinity which concludes reduction of ferredoxin expression. For this reduction, decrease in activity and decrease in metronidazole drug delivery into the cells occur. Mutations in these four isolates may lead to resistance of them to metronidazole.

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How to Cite
1.
Heidari S, Bandehpour M, Seyyed-Tabaei S-J, Valadkhani Z, Haghighi A, Abadi A, Kazemi B. Ferredoxin Gene Mutation in Iranian Trichomonas vaginalis Isolates. IJPA. 8(3):402-7.
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