Iranian Journal of Parasitology 2014. 9(1):20-27.

Genotyping of Echinococcus granulosus Isolates from Human Clinical Samples Based on Sequencing of Mitochondrial Genes in Iran, Tehran.
Bahram Nikmanesh, Hossein Mirhendi, Zohreh Ghalavand, Masoud Alebouyeh, Mitra Sharbatkhori, EshratBeigom Kia, Mehdi Mohebali, Maryam Eghbali, Mohammad Bagher Rokni

Abstract


 

Background: The present study was aimed to investigate molecular diversity of Echino-coccus granulosus isolates collected from human clinical samples using two mitochondrial genes cox1 and nad1 in Iran.

Methods: Forty seven human hydatid cysts were collected through surgery from two hospitals in Tehran during 2010-2012. To determine the fertility of protoscoleces, the cyst fluids were subjected to morphological microscopic examinations. Protoscoleces were removed from each cyst and their total genomic DNAs were extracted. PCR was performed to amplify fragments of 450 and 400 base pair (bp) for cox1 and nad1 genes, respectively. Genotype diversity and sequence variation of the strains were studied by bioinformatics software and in comparison with those mtDNA sequences already de-posited in GenBank.

Results: Sixteen, (53.3%), 13 (43.3%), and 1 (3.3%) samples were related to lung, liver, and spleen, respectively. The remained 17 unfertile samples were excluded from the study. From the 29 isolates, 86.7% (n=26) and 10% (n=3) were related to G1, and G3 genotypes, respectively. The sole isolate with G6 genotype was obtained from lung sample. Analysis of concatenated sequences of cox1+nad1 indicated the presence of 11 haplotypes among our strains that were related to genotypes G1 (n=9), G3 (n=1) and G6 (n=1).

Conclusion: In consistent to other reports from Iran, genotypes G1, G3, and G6 were observed in our human isolates. The rate of G3 genotype was however higher than other studies implying that human can be considered as a new appropriate host for G3 genotype. Further studies with more sample size from different geographic areas of Iran are needed for E. granulosus mapping.

Keywords


Echinococcus granulosus; Genotyping; Iran; cox1; nad1

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References


Budke CM, Deplazes P, Torgerson PR. Global socioeconomic impact of cystic echinococ-cosis. Emerg Infec Dis. 2006; 12(2):296–303.

Rostami Nejad M, Jahani-Sherafat S, Cheraghi-pour K, NazemallhoseiniMojarad E, Taghi-pour N, Zali MR. Hydatic cyst prevalence inslaughtered animals, A neglected health prob-lem. Paramed Sci J.2012; 3(3):25-29.

Sadjjadi SM. Present situation of echinococ-cosis in the Middle East and Arabic North Af-rica. Parasitol Int. 2006. 55 Suppl: S197-202.

Battelli G, Mantovani A, Seimenis A. Cystic Echinococcosis and the Mediterranean Region: a long-lasting association. Parasitol. 2000; 44:43–57.

Ahmadi N, Dalimi A. Characterization of Echi-nococcus granulosus isolates from human, sheep and camel in Iran. Infect Genet Evol. 2006; 6(2):85-90.

Rokni MB. Echinococcosis /hydatidosis in Iran. Iranian J Parasitol. 2009; 4:1-16.

RostamiNejad M, NazemalhosseiniMojarad E, Nochi Z, FasihiHarandi M, Cheraghipour K, Mowlavi GR, et al. Echinococcus granulosus strain differentiation in Iran based on sequence het-erogeneity in the mitochondrial 12S rRNA gene. J Helminthol.2008; 82(4):343-7.

RostamiNejad M, NazemalhosseiniMojarad E, FasihiHarandi M. Echinococcosis: based on molecular studies in Iran. Gastroenterol Hepa-tol Bed Bench. 2010; 3:169–176.

Bowles J, McManus DP. Genetic characteriza-tion of the Asian Taenia, a newly described Tae-niidcestode of humans. Am J Trop Med Hyg. 1994; 50:33–44.

Bowles J, Blair D, McManus DP. Genetic vari-ants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol.1992; 54:165–173

Bowles J, Blair D, McManus DP. Molecular genetic characterization of the cervid strain („northern form‟) of Echinococcus granulosus. Para-sitol.1994; 109:215–221.

Craig PS, Rogan MT, Campos-Ponce M. Echi-nococcosis: disease, detection and transmis-sion. Parasitology.2003; 127:5-20.

Sharbatkhori M, FasihiHarandi M, Mirhendi H, Hajialilo E, Kia EB. Sequence analysis of cox1 and nad1 genes in Echinococcus granulosus G3 genotype in camels (Camelusdromedarius) from central Iran. Parasitol Res. 2011; 108(3):521-7.

Amin Pour A, Hosseini SH, Shayan P. Com-parative genotyping of Echinococcus granulosus in-fecting buffalo in Iran using cox1 gene. Par-asitol Res. 2011; 108:1229–1234.

Zhang L, Eslami A, Hosseini SH, McManus DP. Indication of the presence of two distinct strains of Echinococcus granulosus in Iran by mito-chondrial DNA markers. Am J Trop Med Hyg. 1998; 59:171-174.

Harandi MF, Hobbs RP, Adams PJ, Mobedi I, Morgan-Ryan UM, Thompson RCA. Molecu-lar and morphological characterization of Echi-nococcus granulosusof human and animal origin in Iran. Parasitol. 2002; 125:367–373.

RostamiNejad M, Taghipour N, Nochi Z, NazemalhosseiniMojarad E, Mohebbi SR, FasihiHarandi M, et al. Molecular identification of animal isolates of Echinococcus granulosus from Iran using four mitochondrial genes. J Helmin-thol. 2012; 86(4):485-492.

Borji H, Azizzadeh M, Kamelli M. A retrospec-tive study of abattoir condemnation due to parasitic infections: economic importance in Ahwaz, southwestern Iran. J Parasitol. 2012; 98(5): 954-957.

Dinkel A, Njoroge EM, Zimmermann A, Walz M, Zeyhle E, Elmahdi IE, et al. A PCR system for detection of species and genotypes of the Echinococcus granulosus complex, with reference to the epidemiological situation in eastern Af-rica. Int J Parasitol. 2004; 34:645–653.

Maillard S, Benchikh-Elfegoun M, Knapp J, Bart J, Koskei P, Gottstein B, et al. Taxonomic position and geographical distribution of the common sheep G1 and camel G6 strains of Echinococcus granulosus in three African countries. Parasitol Res. 2007; 100:495–503.

Sadjjadi SM, Mikaeili F, Karamian M, Maraghi S, Sadjjadi FS, Shariat-Torbaghan S, et al. Evi-dence that the Echinococcus granulosus G6 geno-type has an affinity for the brain in humans. Int J Parasitol. 2013; (In Press).

Khademvatan S, Yousefi E, Rafiei A, Rahdar M, Saki J. Molecular characterization of live-stock and human isolates of Echinococcus granu-losus from south-west Iran. J Helminthol. 2013; 87(2):240-4.

Gholami Sh, Sosari M, Fakhar M, Sharif M, Daryani A, Hashemi M, et al. Molecular Char-acterization of Echinococcus granulosus from Hy-datid Cysts Isolated from Human and Animals in Golestan Province, North of Iran. Iranian J Parasitol. 2012; 7(4):8-16.

Shahnazi M, Hejazi H, Salehi M, Andalib AR. Molecular characterization of human and ani-mal Echinococcus granulosus isolates in Isfahan, Iran. Acta Trop. 2011; 117(1):47-50.

Sharbatkhori M, Mirhendi H, Jex AR, Pangasa A, Campbell BE, Kia EB, et al. Genetic catego-rization of Echinococcus granulosus from humans and herbivorous hosts in Iran using an inte-grated mutation scanning-phylogenetic ap-proach. Electrophoresis. 2009; 30(15):2648-55.

Pezeshki A, Akhlaghi L, Sharbatkhori M, Razmjou E, Oormazdi H, Mohebali M, et al. Genotyping of Echinococcus granulosus from do-mestic animals and humans from Ardabil Province, northwest Iran. J Helminthol. 2012; 10:1-5.

Gholami Sh, Sosari M, Fakhar M, Sharif M, Daryani A, Hashemi M, et al. Molecular Char-acterization of Echinococcus granulosus from Hy-datid Cysts Isolated from Human and Animals in Golestan Province, North of Iran. Iranian J Parasitol. 2012; 7(4):8-16.

Dousti M, Abdi J, Bakhtiyari S, Mohebali M, Mirhendi Sh, Rokni MB. Genotyping of Hyda-tid Cyst Isolated from Human and Domestic Animals in Ilam Province, Western Iran Using PCR-RFLP. Iranian J Parasitol. 2013; 8(1):47-52.

Khademvatan S, Yousefi E, Rafiei A, Rahdar M, Saki J. Molecular characterization of live-stock and human isolates of Echinococcus granu-losus from south-west Iran. J Helminthol. 2013; 87(2):240-4.

McManus DP. The molecular epidemiology of Echinococcus granulosus and cystic hydatid disease. Trans R Soc Trop Med Hyg. 2002; 96(Suppl. 1):151e7.

McManus DP, Thompson RC (2003). Molecu-lar epidemiology of cystic echinococcosis. Par-asitology.2003; 127(Suppl. 1): 37e51.


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