Trichostrongylus colubriformis: Possible Most Common Cause of Human Infection in Mazandaran Province, North of Iran.
AbstractBackground: Infection with Trichostrongylus spp. is common among human and herbivorous in most parts of Iran, especially in southern and northern ar-eas. The aim of present study was to identify Trichostrongylus spp. among hu-man population using excreted egg specimens, by the molecular method, in Mazandaran Province, northern Iran. Methods:Overall, 33 positive fecal specimens were randomly sampled and examined. PCR amplification of ITS2-rDNA region was performed on the isolated egg and then a restriction fragment length polymorphism (RFLP) profile was considered to discriminate of Trichostrongylus spp.Results:A total of 33 positive fecal specimens, 29(78.9%), 4(12.1%) were found T. colubriformis and T. axei respectively. Our data appear the molecular evidence of both human T. colubriformis and T. axei infections in North of Iran.Conclusion:T. colubriformis was the probable most common zoonotic species caus-ing human trichostrongylosis infection in the area
Beaver PC, Jung RC, Cupp EW. Clinical Para-sitology. Philadelphia, PA : Lea and Febiger 1984; p 289 – 291.
Boreham RE, Mc Cowan MJ, Ryan AE, All-worth AM, Robson JM. Human trichostron-gyliasis in Queensland. Pathology. 1995; 27(2): 182 – 185.
Ghadirian E, Arfaa F. Present status of tricho-strongyliasis in Iran. Am J Trop Med Hyg. 1975; 24(6): 935 – 941.
Phosuk I, Intapan PM, Sanpool O, Janwan P, Thanchomnang T, Sawanyawisuth K, et al. Mo-lecular Evidence of Trichostrongylus colubriformis and Trichostrongylus axei Infections in Humans from Thailand and Lao PDR. Am J Trop Med Hyg. 2013; 89(2):376-9.
Yong TS, Lee JH, Sim S, Lee J, Min DY, Chai JY, et al. Differential diagnosis of Trichostrongylus and hookworm eggs via PCR using ITS-1 se-quence. Korean J Parasitol. 2007; 45(1): 69 -74.
Ghadirian E. Human infection with Trichostron-gylus lerouxi (Biocca, Chabaud, and Ghadirian, 1974) in Iran. Am J Trop Med Hyg. 1977; 26(6):1212-3.
Shahbazi A, Fallah E, Kohansal Koshki M H, Nematollahi A, Ghazanchaei A, Asfaram Sh. Morphological characterization of the Trichostrongylus species isolated from sheep in Tabriz, Iran. Res J Vet Sci. 2012; 2(5):309-12.
Ghasemikhah R, Mirhendi H, Kia EB, Mowlavi Gh, Sarmadian H, Meshgi B, et al. Morpho-logical and Morphometrical Description of Tri-chostrongylus Species Isolated from Domestic Ruminants in Khuzestan Province, Southwest Iran. Iran J Parasitol. 2011; 6(3): 82-88.
Pestechian N, Baghaei M, Yosefi H. Study on Trichostrongylus eggs isolated from sheep by PCR-RFLP in Isfahan. J Feyz. 2005; 35: 23-29 (In Persian).
Ghasemikhah R, Sharbatkhori M, Mobedi I, Kia EB, Fasihi Harandi M, Mirhendi H. Se-quence Analysis of the Second Internal Tran-scribed Spacer (ITS2) Region of rDNA for Spe-cies Identification of Trichostrongylus Nematodes Isolated From Domestic Livestock in Iran. Iran J Parasitol. 2012; 7(2): 40–46.
Gasser RB, Chilton NB, Hoste H, Stevenson LA. Species Identification of Trichostrongyle Nematodes by PCR-Linked RFLP. Int J Parasitol. 1994;24(2): 291-3.
Schnieder T HM, Epe C. Genus-specific PCR for the differentiation of eggs or larvae from gastrointestinal nematodes of ruminants. Parasitol Res. 1999;85:895-8.
Baghaei MPN, Salehi M, Sadeghi H. Identification of Trichostrongylus Nematodes by
PCR-RFLP Technique in Isfahan. J Isfahan Med Sch. 2005;23(77):42-8 (In Persian).
Eslami A RS, Nikbin S. Gastrointestinal nematodes of gazelle,Gazella subguttrosa in Iran. Vet Parasitol. 1980;7:75-8.
Gholami Sh, Sharif M, Mobedi I, Ziaei H, Mo-hammadpour R, Kyanyan H. Intestinal Proto-zoan Infections in cattle breeders in rural re-gions of Mazandaran province during 2003. J Mazand Univ Med Sci. 2005; 45(14): 51-60 (In Persian).
Sato M, Yoonuan T, Sanguankiat S, Nuamta-nong S, Pongvongsa T, Phimmayoi I et al. Hu-man Trichostrongylus colubriformis Infection in a Rural Village in Laos. Am J Trop Med Hyg. 2011; 84(1):52-4.
Hoste H, Chilton NB, Gasser RB, Beveridge I. Differences in the second internal transcribed spacer (ribosomal DNA) between five species of Trichostrongylus (Nematoda: Trichostrongyli-dae). Int J Parasitol.1995; 25(1):75–80.
Hoste H, Gasser RB, Chilton NB, Mallet S, Beveridge I. Lack of intraspecific variation in the second internal transcribed spacer (ITS-2) of Trichostrongylus colubriformis ribosomal DNA. Int J Parasitol.1993; 23(8):1069–71.
Rokni M B. The present status of human hel-minthic diseases in Iran. Ann Trop Med Para-sitol. 2008; 102(4): 283–295.
Ghadirian E, Arfaa F, Sadighian A. Human infection with Trichostrongylus capricola in Iran. Am J Trop Med Hyg. 1974; 23(5):1002-3.
Sayyari AA, Imanzadeh F, Bagheri -Yazdi S A, Karami H, Yaghoobi M. Prevalence of intesti-nal parasitic infections in the Islamic Republic of Iran. East Mediterr Health J. 2005; 11:377–383.
Gasser RB. Chilton NB, Hoste1 H, Beveridge I. Rapid sequencing of rDNA from single worms and eggs of parasitic helminthes. Nucl Acid Res. 1993; 21 (10) : 2525-26.