Iranian Journal of Parasitology 2015. 10(1):110-115.

Trichostrongylus colubriformis: Possible Most Common Cause of Human Infection in Mazandaran Province, North of Iran.
Shirzad Gholami, Farhang Babamahmoodi, Rohallah Abedian, Mehdi Sharif, Abbas Shahbazi, Abdolsattar Pagheh, Mahdi Fakhar



Background: Infection with Trichostrongylus spp. is common among human and herbivorous in most parts of Iran, especially in southern and northern ar-eas. The aim of present study was to identify Trichostrongylus spp. among hu-man population using excreted egg specimens, by the molecular method, in Mazandaran Province, northern Iran.

Methods:Overall, 33 positive fecal specimens were randomly sampled and examined. PCR amplification of ITS2-rDNA region was performed on the isolated egg and then a restriction fragment length polymorphism (RFLP) profile was considered to discriminate of Trichostrongylus spp.

Results:A total of 33 positive fecal specimens, 29(78.9%), 4(12.1%) were found T. colubriformis and T. axei respectively. Our data appear the molecular evidence of both human T. colubriformis and T. axei infections in North of Iran.

Conclusion:T. colubriformis was the probable most common zoonotic species caus-ing human trichostrongylosis infection in the area


Human; ITS2-PCR; Iran; Trichostrongylus axei; Trichostrongylus colubriformis

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