Iranian Journal of Parasitology 2018. 13(2):186-192.

Comparison of p27 Gene Expression of Promastigote and Amastigote Forms of Leishmania major (MRHO/IR/75/ER) by Real-time RT-PCR
Samira ELIKAEE, Mehdi MOHEBALI, Hamid ESLAMI, Sassan REZAEI, Hamid Reza NAJAFIAN, Elham KAZEMI-RAD, Hossein KESHAVARZ, Mohammad Reza ESHRAGHIAN, Homa HAJJARAN, Mohammad Ali OSHAGHI, Sara AYAZIAN MAVI

Abstract


Background: Cutaneous leishmaniasis (CL) is one of the world health problems. Leishmania major is the etiological agent of zoonotic cutaneous leishmaniasis (ZCL). Promastigote and amastigote are two morphological forms of Leishmania parasites that express different proteins and p27 is an important gene encoding cytochrome c oxidase (COX) component. P27 gene expresses a 27 kDa protein that essential in ATP synthesis. This study aimed to compare p27 gene expression in promastigote and amastigote forms in Iranian strain of L. major (MRHO/IR/75/ER).

Methods: This study was conducted in 2015. Clinical isolates of CL patients from north, center, west and south parts of Iran were collected and identified by PCR-RFLP. After RNA extraction of promastigotes and amastigotes and cDNA synthesis, the expression level of p27 gene was compared by real-time RT-PCR.

Results: By comparison of expression level between amastigote and promastigote forms of Iranian strain of L. major, up-regulation of p27 gene (2.73 fold) was observed in amastigotes. Moreover, there was no significant difference in p27 gene expression between L. major isolates.

Conclusion: p27 gene and protein can be considered as a target in recombinant vaccine production and treatment process.


Keywords


p27 gene, Real-time RT-PCR, Leishmania major, Amastigote, Promastigote

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