Genetic Characterization of Hydatid Cysts Isolated from Do-mestic Animals in Lorestan Province, Western Iran
AbstractBackground: Regarding hydatid cyst (cystic echinococcosis, CE) as a human public health problem in the West of Iran, molecular data related to the genotypes of Echinococcus granulosus in cattle and sheep in these regions are still insufficient. Here, we evaluated the genotypes of E. granulosus infecting sheep and cattle in western Iran.Methods: Totally, 36 hydatid cysts including 18 hydatid cysts of sheep and 18 hydatid cysts of cattle were collected from Khorramabad slaughterhouse (Lorestan Province), Western Iran between May to September 2014. Protoscoleces or germinal layers were collected from individual cysts, DNA was extracted, and genotyping was performed by sequencing and analyzing mitochondrial cytochrome c oxidase subunit 1 (cox1) gene.Results: In sequencing analysis, all of sheep isolates belonged to genotype G1 (sheep strain). Among cattle hydatid cyst isolates, 16/18 (88.9%) were belonged to genotype G1 and 2/18 (11.1%) were belonged to G3 genotype. The phylogenetic analysis showed two clusters; one of the clusters includes cattle G3 genotype and the other cluster represents sheep and cattle G1 genotype that were isolated.Conclusion: The common sheep strain/G1 is predominant genotype in the western part of Iran, followed by G3 genotype, circulating among the animal hosts in this region. Further studies covering a larger number of isolates might be necessary to see if there are other genotypes in the hydatid cyst population in this region of Iran.
World Health Organization (WHO) in-formal working group on echinococcosis. Bull WHO.1996; 74:231-42.
Mahmoudvand H, Kheirandish F, Dezaki ES, Shamsaddini S, Harandi MF. Chemical composition, efficacy and safety of Pistacia vera (var. Fandoghi) to inactivate protosco-leces during hydatid cyst surgery. Biomed Pharmacother. 2016; 82: 393-8.
Schneider R, Gollackner B, Schindl M, Tucek G, Auer H. Echinococcus canadensis G7 (pig strain): an underestimated cause of cystic echinococcosis in Austria. Am J Trop Med Hyg. 2010; 82(5):871-4.
Thompson RC. The taxonomy, phylogeny and transmission of Echinococcus. Exp Para-sitol. 2008; 119(4):439-46.
Rostami Nejad M, Taghipour N, Nochi Z, Mojarad EN, Mohebbi SR, Harandi MF, Zali MR. Molecular identification of animal isolates of Echinococcus granulosus from Iran using four mitochondrial genes. J Helmin-thol. 2012; 86(4):485-92.
Sharbatkhori M, Mirhendi H, Jex AR, Pangasa A, Campbell BE, Kia EB, Eshraghian MR, Harandi MF, Gasser RB. Genetic categorization of Echinococcus granu-losus from humans and herbivorous hosts in Iran using an integrated mutation scan-ning-phylogenetic approach. Electropho-resis. 2009; 30(15):2648-55.
Sharbatkhori M, Fasihi Harandi M, Mir-hendi H, Hajialilo E, Kia EB. Sequence analysis of cox1 and nad1 genes in Echino-coccus granulosus G3 genotype in camels (Camelus dromedarius) from central Iran. Par-asitol Res. 2011; 108(3):521-7.
Rostami Nejad, M, Nazemalhosseini Mo-jarad E, Fasihi Harandi M. Echinococ-cosis: based on molecular studies in Iran. Gastroenterology Hepatology Bed Bench. 2010; 3: 169-76.
Parsa F, Fasihi Harandi M, Rostami S, Sharbatkhori M. Genotyping Echinococcus granulosus from dogs from Western Iran. Exp Parasitol. 2012; 132(2):308-12.
Bowles J, Blair D, McManus DP. Genetic variants within the genus Echinococcus identi-fied by mitochondrial DNA sequencing. Molecul Biochem Parasitol. 1992; 54(2):165-73.
Kheirandish F, Sharafi AC, Kazemi B, Bandehpour M, Tarahi Mj, Khamesipour A. First molecular identification of Leishma-nia species in a new endemic area of cuta-neous leishmaniasis in Lorestan, Iran. Asian Pac J Trop Med. 2013;6(9):713-7.
Kheirandish F, Chegeni Sharafi A, Kazemi B, Mohebali M, Sarlak A, Tarahi MJ, Holakouee K, Hajaran H. Identification of Leishmania species using PCR assay on Giemsa-stained slides prepared from cu-taneous leishmaniasis patients. Iran J Para-sitol. 2013;8(3):382-8.
Kheirandish F, Badparva E, Haghighi A, Nazemalhosseini Mojarad E, Kazemi B. Differential diagnosis of Entamoeba spp. in gastrointestinal disorder patients in Khorramabad, Iran. African J Microbiol Res. 2011; 5(18): 2863-6.
Kheirandish F, Tarahi MJ, Ezatpour B. Prevalence of intestinal parasites among food handlers in West of Iran. Rev Inst Med Trop Sao Paulo. 2014;56(2):111-4.
Badparva E, Kheirandish F, Ebrahimzade F. Prevalence of intestinal parasites in Lorestan Province, West of Iran. Asian Pac J Trop Dis. 2014; 4: 930-4.
Kheirandish F, Tarahi M, Haghighi A, Nazemalhosseini-Mojarad E, Kheirandish M. Prevalence of intestinal parasites in bakery workers in Khorramabad, Lorestan Iran. Iran J Parasitol. 2011; 6(4): 76-83.
Utuk AE, Simsek S, Koroglu E, McManus DP. Molecular genetic characterization of different isolates of Echinococcus granulosus in east and southeast regions of Turkey. Acta Trop. 2008; 107(2):192-4.
Vural G, Baca AU, Gauci CG, Bagci O, Gicik Y, Lightowlers MW. Variability in the Echinococcus granulosus cytochrome C ox-idase 1 mitochondrial gene sequence from livestock in Turkey and a reappraisal of the G1–3 genotype cluster. Vet Parasitol. 2008; 154(3-4):347-50.
Casulli A, Manfredi MT, La Rosa G et al. Echinococcus ortleppi and E. granulosus G1, G2 and G3 genotypes in Italian bovines.. Vet Parasitol. 2008 Aug 1;155(1-2):168-72.
M’rad S, Oudni-M’rad M, Filisetti D et al. Molecular identification of Echinococcus granulosus in Tunisia: first record of the Buffalo strain (G3) in human and bovine in the country. Open Vet Sci J. 2010; 4:27-30.
de la Rue ML, Takano K, Brochado JF, Costa CV et al. Infection of humans and animals with Echinococcus granulosus (G1 and G3 strains) and E. ortleppi in Southern Bra-zil. Vet Parasitol. 2011; 177(1-2):97-103.
Hajialilo E, Harandi MF, Sharbatkhori M, Mirhendi H, Rostami S. Genetic character-ization of Echinococcus granulosus in camels, cattle and sheep fromthe south-east of Iran indicates the presence of the G3 gen-otype. J Helminthol. 2012; 86(3):263-70.
Pednekar RP, Gatne ML, Thompson RC, Traub RJ. Molecular and morphological characterisation of Echinococcus from food producing animals in India. Vet Parasitol. 2009; 165(1-2):58-65.
Yoosefi H, Hashemzadeh M, Aliyari Z. Molecular study of hydatid cyst (sheep strain) in Chaahrmohal va Bakhteyari by PCR-RFLP. J Shahrkord Uni. 2007; 9(2):28-33.
Shahnazi M, Hejazi H, Salehi M, Andalib AR. Molecular characterization of human and animal Echinococcus granulosus isolates in Isfahan, Iran. Acta Trop. 2011; 117(1):47-50.
Farhadi M, Fazaeli A, Haniloo A. Genetic characterization of livestock and human hydatid cyst isolates from northwest Iran, using the mitochondrial cox1 gene se-quence. Parasitol Res. 2015; 114(12):4363-70.
Yakhchali M, Mardani K. Study on strain variation of Echinococcus granulosus in domes-tic life cycle by amplification of nad-1 gene by PCR-RFLP. Iran J Vet. 2011;7(1):63-8.
Sadri A, Moshfe A, Doosti A. Characteri-zation of isolated hydatid cyst from slaughtered livestock in Yasuj industrial slaughterhouse by PCR-RFLP. J Yasuj Uni. 2012; 17(3):143-52.
Pezeshki A, Akhlaghi L, Sharbatkhori M, Razmjou E, Oormazdi H, Mohebali M, Meamar AR. Genotyping of Echinococcus granulosus from domestic animals and hu-mans from Ardabil Province, northwest Iran. J Helminthol. 2012;87(4):387-91.
Parsa F, Haghpanah B, Pestechian N, Salehi M. Molecular epidemiology of Echi-nococcus granulosus strains in domestic herbi-vores of Lorestan, Iran. Jundishapur J Mi-crobiol. 2011; 4(2): 123-30.
Kheirandish F, Mahmoudvand H, Ah-madinejad M, Karimi Rouzbahani A. Ge-netic characterization of human-derived hydatid cysts of Echinococcus granulosus in Lorestan Province, Western Iran. Tropical Biomed. 2017; 34(4): 863-9.