Iranian Journal of Parasitology 2018. 13(1):89-99.

Trypanocidal Effects of Cisplatin alone and in Combination with Nigella sativa Oil on Experimentally Infected Mice with Trypanosoma evansi
Nashaat Abd El-Monem NASSEF, Manal Ahmed EL-MELEGY, Engy Victor BESHAY, Dalia Rifaat Al-SHARAKY, Tahany Mohamed AL-ATTAR

Abstract


Background: Due to the limited number of the available drugs for the treatment of trypanosomiasis, this study was designed to evaluate the trypanocidal effects of cisplatin or/and Nigella sativa oil (NSO) in experimentally infected mice with T. evansi.

Methods: During 2015 at the Parasitology Department, Menoufia University, Menoufia, Egypt, sixty Swiss albino mice were divided into six groups: normal control (I), infected control (II); cisplatin-treated (III); NSO-treated (IV); combined cisplatin + NSO-treated (V) and diminazene-treated (VI). The tested drugs were evaluated by the assessment of parasitaemia, measurement of aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea, creatinine, serum IgM and a histopathological study.

Results: NSO showed a trypanocidal effect, however; it was not as effective as cisplatin or diminazene. There were significant increases of AST, ALT, urea, and creatinine in group II and III, which were significantly reduced in cisplatin + NSO-treated group (V). Moreover, there were significant reductions in serum IgM and the pathological changes of the examined organs of group V when they were compared with other treated groups.

Conclusion: Cisplatin combined with NSO showed a trypanocidal effect against T. evansi with preservation of vital organs functions and architecture.


Keywords


T. evansi; Mice; Cisplatin; Diminazene; N. sativa; Trypanocidal

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References


Habila N, Inuwa MH, Aimola IA et al. Pathogenic mechanisms of Trypanosoma evansi infections. Res Vet Sci. 2012; 93(1):13-7.

Fernández D, González-Baradat B, Eleizalde M, González-Marcano E, Perrone T, Mendoza M. Trypanosoma evansi: A comparison of PCR and parasitological diagnostic tests in experimentally infected mice. Exp Parasitol. 2009; 121(1):1-7.

Nwodo NJ, Ibezim A, Ntie-Kang F, Adikwu MU, Mbah CJ. Anti-trypanosomal activity of Nigerian plants and their constituents. Molecules. 2015; 20(5):7750-71.

Pessetto ZY, Weir SJ, Sethi G, Broward MA, Godwin AK. Drug repurposing for gastrointestinal stromal tumor. Mol Cancer Ther. 2013; 12(7):1299-309.

Sachdeva H, Sehgal R, Kaur S. Tinospora cordifolia as a protective and immunomodulat-ory agent in combination with cisplatin against murine visceral leishmaniasis. Exp Parasitol. 2014; 137:53-65.

Sachdeva H, Sehgal R, Kaur S. Asparagus racemosus ameliorates cisplatin induced toxicities and augments its anti-leishmanial activity by immunomodulation in vivo. Parasitol Int. 2014; 63(1):21-30.

Entok E, Ustuner MC, Ozbayer C, Tekin N, Akyuz F, Yangi B, Kurt H, Degirmenci I, Gunes HV. Anti-inflammatory and anti-oxidative effects of Nigella sativa L: 18FDG-PET imaging of inflammation. Mol Biol Rep. 2014; 41(5):2827-34.

El-Sheikh AA, Morsy MA, Abdalla AM, Hamouda AH, Alhaider IA. Mechanisms of thymoquinone hepatorenal protection in methotrexate-induced toxicity in rats. Mediators of Inflammation 2015; 2015:859383.

Majdalawieh AF, Fayyad MW. Imm-unomodulatory and anti-inflammatory action of Nigella sativa and thymoquinone: A comprehensive review. Int Immunopharmacol. 2015; 28(1):295-304.

Emeka PM, Badger-Emeka LI, Eneh CM, Khan TM. Dietary supplementation of chloroquine with Nigella sativa seed and oil extracts in the treatment of malaria induced in mice with Plasmodium berghei. Pharmacogn Mag. 2014; 10(Suppl 2):S357-62.

Desquesnes M, Dargantes A, Lai DH, Lun ZR, Holzmuller P, Jittapalapong S. Trypanosoma evansi and Surra: a review and perspectives on transmission, epidemiology and control, impact, and zoonotic aspects. Biomed Res Int. 2013; 2013:321237.

Garcia LS and Bruckner DA. Procedures for detecting blood parasite. In: Garcia LS and Bruckner DA editors, Diagnostic parasitology. Washington D.C. USA: ASM Press; 1997. p. 698-703.

Kumar U, Jas R, Ghosh JD. Effect of Isometamidium hydrochloride on Trypanosoma evansi Infections in Rats. J Parasit Dis. 2009; 33(1-2):36-41.

Wysor MS, Zwelling LA, Sanders JE, Grenan MM. Cure of mice infected with Trypanosoma rhodesiense by cis-diamminedichloroplatinum (II) and disulfiram rescue. Science. 1982; 217(4558):454-6.

Paim FC, Duarte MM, Costa MM, Da Silva AS, Wolkmer P, Silva CB, Paim CB, França RT, Mazzanti CM, Monteiro SG, Krause A, Lopes ST. Cytokines in rats experimentally infected with Trypanosoma evansi. Exp Parasitol. 2011; 128(4):365-70.

Ferreira Neto, J.M., Viana, E.S., Magalháes, L.M. Patologia Clínica Veterinária. Belo Horizonte, Rabelo; 1981. p.279.

Drury RB and Wallington EA. Carleton's Histological Technique, 5th ed. New York, USA: Oxford University Press; 1980. p.270.

Navarro M, Marchan E, Maldonado A, Simoni Z, Velásquez M, Herrera L, Visbal G. Anti-trypanosomatid activity of platinum–sterol hydrazone imidazoline complexes. Transition Met Chem. 2015; 40:707–713.

Mady RF, El-Hadidy W, Elachy S. Effect of Nigella sativa oil on experimental toxoplasmosis. Parasitol Res. 2016; 115(1):379-90.

Sharma M, Sehgal R, Kaur S. Evaluation of nephroprotective and immunomodulatory activities of antioxidants in combination with cisplatin against murine visceral leishmaniasis. PLoS Negl Trop Dis. 2012; 6(5):e1629.

Al-Malki AL, Sayed AA. Thymoquinone attenuates cisplatin-induced hepatotoxicity via nuclear factor kappa-β. BMC Complement Altern Med. 2014; 14:282.

Gilani AH, Jabeen Q and Khan MA. A review of medicinal uses and pharmacological activities of Nigella sativa. Pak J Biol Sci. 2004; 7: 441-451.

Hadjzadeh MA, Keshavarzi Z, Tabatabaee Yazdi SA, Ghasem Shirazi M, Rajaei Z, Khajavi Rad A. Effect of alcoholic extract of Nigella Sativa on cisplatin induced toxicity in rat. Iran J Kidney Dis. 2012; 6(2):99-104.

Aquino LP, Machado RZ, Alessi AC, Marques LC, de Castro MB, Malheiros EB. Clinical, parasitological and immunological aspects of experimental infection with Trypanosoma evansi in dogs. Mem Inst Oswaldo Cruz. 1999; 94(2):255-60.

Baral TN, De Baetselier P, Brombacher F, Magez S. Control of Trypanosoma evansi infection is IgM mediated and does not require a type I inflammatory response. J Infect Dis. 2007; 195(10):1513-20.

Biswas D, Choudhury A, Misra KK. Histopathology of Trypanosoma (Trypanozoon) evansi infection in Bandicoot Rat. I. Visceral organs. Exp Parasitol. 2001; 99(3):148-59.

Mbaya A, Kumshe H, Nwosu CO. The Mechanisms of Anemia in Trypanosomiasis. A Review. In: Silverberg D editor. Anemia. InTech; 2012, p. 269-82.

Cadioli FA, Marques LC, Machado RZ, Alessi AC, Aquino LPCT, Barnabé PA. Experimental Trypanosoma evansi infection in donkeys: hematological, biochemical and histopathological changes. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia. 2006; 58(5): 749-756.

Rodrigues A, Fighera RA, Souza TM, Schild AL, Barros CS. Neuropathology of Naturally Occurring Trypanosoma evansi Infection of Horses. Vet Pathol. 2009; 46(2):251-8.

Dkhil MA, Al-Quraishy S, Aref AM, Othman MS, El-Deib KM, Abdel Moneim AE. The potential role of Azadirachta indica treatment on cisplatin-induced hepatotoxicity and oxidative stress in female rats. Oxid Med Cell Longev. 2013; 2013:741817.

Baldissera MD, Gonçalves RA, Sagrillo MR et al. Effects of treatment with the anti-parasitic drug diminazene aceturate on antioxidant enzymes in rat liver and kidney. Naunyn Schmiedebergs Arch Pharmacol. 2016; 389(4):429-38.

Peregrine AS, Mamman M. Pharmacology of diminazene: a review. Acta Trop. 1993; 54(3-4):185-203.

Onyeyili PA, Anika SM. Diminazene aceturate residues in the tissues of healthy, Trypanosoma congolense and Trypanosoma brucei brucei infected dogs. Br Vet J. 1991; 147(2):155-62.

Namikawa K, Asakura M, Minami T, Okazaki Y, Kadota E, Hashimoto S. Toxicity of cisplatin to the central nervous system of male rabbits. Biol Trace Elem Res. 2000; 74(3):223-35.


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