Iranian Journal of Parasitology 2017. 12(4):614-621.

Genotyping Echinococcus granulosus from Canine Isolates in Ilam Province, West of Iran
Abdolhossein DALIMI, Morteza SHAMSI, Afra KHOSRAVI, Fatemeh GHAFFARIFAR

Abstract


Abstract

Background: Cystic Echinococcosis (CE) is one of the most common parasitic zoonosis caused by Echinococcus granulosus worldwide. This study investigated genotype diversity of Echinococcus granulosus isolated from stray dogs and golden jackals in Ilam province, West of Iran.

Methods: Adult worms were collected from the small intestine of the stray dogs and golden jackals from Ilam Province roads during 2012-2014. DNA was extracted from the adult worms and the partial mitochondrial NADH dehydrogenase subunit1 (nad1) was amplified by PCR then the products were digested by using HpaII, Rsa1, and Alu1 restriction enzymes. In order to confirm RFLP results, a number of PCR products were bi-directionally sequenced.

Results: Totally, 20 stray dogs out of 75 (26.66%) and two out of 73 (2.74%) golden jackal showed infection with E. granulosus. Amplified PCR product for all isolates was a band of approximately 550bp. Alu1 digested the product into two bands of approximately 160bp and 390bp fragments, while the Rsa1 cut the product into 320bp and 230bp fragments and the HpaII had no effect on the PCR product for both dog and jackal samples. The isolate sequences of mtDNAnad1 gene indicated 100% homology with references G1, G2 and G3 sequences in the GenBank database.

Conclusion: The genotype of adult E. granulosus was similar to larval stage genotypes of parasite (G1-G3 complex).


Keywords


Echinococcus granulosus, Stray dog, Golden jackal, Genotyping, Iran

Full Text:

PDF

References


McManus DP, Thompson RC. Molecular epidemiology of cystic Echinococcosis. Parasitology.2003;127 Suppl:S37-51.

Heidari Z, Mohebali M, Zarei Z et al. Seroepidemiological study of human hydatidosis in Meshkinshahr district, ardabil province, Iran. Iran J Parasitol. 2011;6(3):19-25.

Rokni MB. Echinococcosis /hydatidosis Iran. Iran J Parasitol.2009;4(1):1-16.

Thompson RC. The taxonomy, phylogeny and transmission of Echinococcus. Exp Parasitol. 2008;119(4):439-46.

Nakao M, McManus DP, Schantz PM, Craig PS, Ito A. A molecular phylogeny of the genus Echinococcus inferred from complete mitochondrial genomes. Parasitology. 2007;134(Pt 5):713-22.

Moro PL, Nakao M, Ito A, Schantz PM, Cavero C, Cabrera L. Molecular identification of Echinococcus isolates from Peru. Parasitol Int. 2009;58(2):184-6.

Utuk AE, Simsek S, Koroglu E, McManus DP. Molecular genetic characterization of different isolates of Echinococcus granulosus in east and southeast regions of Turkey. Acta Trop. 2008;107(2):192-4.

Eckert J, Thompson RC. Intra specific variation of Echinococcus granulosus and related species with emphasis on their infectivity to humans. Acta Trop.1997; 64(1-2):19-34.

Rosenzvit MC, Zhang LH, Kamenetzky L, Canova SG, Guarnera EA, McManus DP. Genetic variation and epidemiology of Echinococcus granulosus in Argentina. Parasitology.1999;118 ( Pt 5):523-30.

Bowles J, McManus DP. Rapid discrimination of Echinococcus species and strains using a polymerase chain reaction based RFLP method. Mol Biochem Parasitol.1993;57(2):231-9.

Brody JR, Kern SE. Sodium boric acid: a Tris-free, cooler conductive medium for DNA electrophoresis. Biotechniques. 2004;36(2):214-6.

Bowles J, Blair D, McManus DP. Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol. 1992;54(2):165-73.

Dalimi A, Sattari A, Motamedi G. A study on intestinal helminthes of dogs, foxes and jackals in the western part of Iran. Vet Parasitol. 2006;142(1-2):129-33.

Abdi J, Panahi J, Havasian M, Sayehmiri K.The prevalence of Echinococcus granulosus in carnivores in Iran: A systematic review. Lab J. 2014;8(5):1-6.

Eslami A, Hosseini SH. Echinococcus granulosus infection of farm dogs of Iran. Parasitol Res. 1998;84(3):205-7.

Harandi MF, Hobbs RP, Adams PJ, Mobedi I, Morgan-Ryan UM, Thompson RC. Molecular and morphological characterization of Echinococcus granulosusof human and animal origin in Iran. Parasitology. 2002;125(Pt 4):367-73.

Dousti M, Abdi J, Bakhtiyari S, Mohebali M, Mirhendi Sh, Rokni M. Genotyping of hydatid cyst isolated from human and domestic animals in Ilam Province, Western Iran Using PCR-RFLP. Iran J Parasitol. 2013;8(1):47-52.

Ahmadi N, Dalimi A. Characterization of Echinococcus granulosus isolates from human, sheep and camel in Iran. Infect Genet Evol. 2006;6(2):85-90.

Parsa F, Haghpanah B, Pestechian N, Salehi M. Molecular epidemiology of Echinococcus granulosus strains in domestic herbivores of Lorestan, Iran. Jundishapur J Microbiol 2011;4(2):123-30.

Zhang L, Eslami A, Hosseini SH, McManus DP. Indication of the presence of two distinct strains of Echinococcus granulosus in Iran by mitochondrial DNA markers. Am J Trop Med Hyg. 1998;59(1):171-4.

Rostami Nejad M, Taghipour N, Nochi Z et al. Molecular identification of animal isolates of Echinococcus granulosus from Iran using four mitochondrial genes. J Helminthol. 2012; 86(4):485-92.

Pezeshki A, Akhlaghi L, Sharbatkhori M, Razmjou E, Oormazdi H, Mohebali M, Meamar AR. Genotyping of Echinococcus granulosus from domestic animals and humans from Ardabil Province, northwest Iran. J Helminthol.2013;87(4):387-91.

Parsa F, Fasihi Harandi M, Rostami S, Sharbatkhori M. Genotyping Echinococcus granulosus from dogs from Western Iran. Exp Parasitol. 2012;132(2):308-12.

Khademvatan S, Yousefi E, Rafiei A, Rahdar M, Saki J. Molecular characterization of livestock and human isolates of Echinococcus granulosus from southwest Iran. J Helminthol. 2013;87(2):240-4.

Sadri A, Moshfe A, Doosti A. Characterization of isolated hydatid cyst from slaughtered livestock in Yasuj industrial slaughterhouse by PCR-RFLP. J Yasuj Uni. 2012;17(3):143-52.

Sharbatkhori M, Mirhendi H, Jex AR et al. Genetic categorization of Echinococcus granulosus from humans and herbivorous hosts in Iran using an integrated mutation scanning-phylogenetic approach. Electrophoresis. 2009;30(15):2648-55.

Sharbatkhori M, Mirhendi H, Harandi MF et al. Echinococcus granulosus genotypes in livestock of Iran indicating high frequency of G1 genotype in camels. Exp Parasitol. 2010; 124(4):373-9.

Sharbatkhori M, Fasihi Harandi M, Mirhendi H, Hajialilo E, Kia EB. Sequence analysis of cox1 and nad1 genes in Echinococcus granulosus G3 genotype in camels (Camelus dromedarius) from central Iran. Parasitol Res. 2011; 108(3):521-7.

Kia EB, Rahimi H, Sharbatkhori M, Talebi A, Fasihi Harandi M, Mirhendi H. Genotype identification of human cystic echinococcosis in Isfahan, central Iran. Parasitol Res. 2010;108(3):521-7.

Hajialilo E, Harandi MF, Sharbatkhori M, Mirhendi H, Rostami S. Genetic characterization of Echinococcus granulosus in camels, cattle and sheep from the south-east of Iran indicates the presence of the G3 genotype. J Helminthol. 2012;86(3):263-70.

Sharifiyazdi H, Oryan A, Ahmadnia S, Valinezhad A. Genotypic characterization of Iranian camel (Camelus dromedarius) isolates of Echinoccocus granulosus. J Parasitol. 2011; 97(2):251-5.

Gholami S, Jahandar H, Abastabar M, Pagheh A, Mobedi I, Sharbatkhori M. Echinococcus granulosus Sensu Stricto in Dogs and Jackals from Caspian Sea Region, Northern Iran . Iran J Parasitol. 2016;11(2):186-194.

Shariatzadeh SA, Spotin A, Gholami S et al. The first morphometric and phylogenetic perspective on molecular epidemiology of Echinococcus granulosus sensu lato in stray dogs in a hyperendemic Middle East focus, northwestern Iran. Parasit Vectors. 2015; 8:409.

Gholami Sh, Sosari M, Fakhar M, Sharif M, Daryani A, Hashemi M, Vahadi M. Molecular characterization of Echinococcus granulosus from hydatid cysts isolated from human and animals in Golestan Province, North of Iran. Iran J Parasitol. 2012;7(4):8-16.

Farhadi M, Fazaeli A, Haniloo A. Genetic characterization of livestock and human hydatid cyst isolates from northwest Iran, using the mitochondrial cox1 gene sequence. Parasitol Res. 2015;114(12):4363-70.

Shahnazi M, Hejazi H, Salehi M, Andalib AR. Molecular characterization of human and animal Echinococcus granulosus isolates in Isfahan, Iran. Acta Trop. 2011;117(1):47-50.

Rostami Nejad M, Nazemalhosseini Mojarad E, Nochi Z et al. Echinococcus granulosus strain differentiation in Iran based on sequence heterogeneity in the mitochondrial 12S rRNA gene. J Helminthol. 2008 82(4):343-7.

Pour AA, Hosseini SH, Shayan P. Comparative genotyping of Echinococcus granulosus infecting buffalo in Iran using cox1 gene. Parasitol Res. 2011;108(5):1229-34.

Nikmanesh B, Mirhendi H, Ghalavand Z et al. Genotyping of Echinococcus granulosus isolates from Human clinical samples based on sequencing of mitochondrial genes in Iran, Tehran. Iran J Parasitol. 2014;9(1):20-7.

Rostami S, Shariat Torbaghan S, Dabiri S, Babaei Z, Ali Mohammadi M, Sharbatkhori M, Fasihi Harandi M. Genetic characterization of Echinococcus granulosus from a large number of formalin-fixed, paraffin-embedded tissue. Am J Trop Med Hyg. 2015;92(3):588-94.


Refbacks

  • There are currently no refbacks.


Creative Commons Attribution-NonCommercial 3.0

This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.