Seroprevalence of Neospora caninum Infection and Associated Risk Factors in Cattle of Sistan Areas, Southeastern Iran in 2016
AbstractBackground: Neospora caninum is protozoan parasitic disease now described as the major cause of abortion and other reproductive issues. The aim of this study was to determine the seroprevalence of N. caninum in cattle breeds of the Sistan region, southeastern border area of Iran. Methods: Using an ELISA kit (ID.VET, France), the antibodies against N. caninum in cattle of Sistan was evaluated in 2016. Overall, 184 blood samples from apparently healthy cattle in the eastern border area of Iran Sistan were collected for assessment of antibodies against N. caninum. The values greater than or equal to 50%, were considered positive based on manufacture’s manual for ELASA kit. Results: 3.8% of 184 cattle have antibody against N. caninum. Chi-square test showed that the seroprevalence among Holsteins, Sistan and cross-breed was 9.4%, 0%, and 4.3%, respectively. No significant difference was observed among the breeds (P>0.05). The seroprevalence was decreased as the age of cow increased and there is no significant difference between the prevalence of N. caninum and different city area. No statistically significant relationship between the seroprevalence of N. caninum and history of abortion, lactation number and infertility was observed. Although there was no significant difference between the cattle breeds of the Sistan region all the Sistani cows were negative for the antibody against N. caninum. Conclusion: Infection rate with N. caninum in bovine population in Sistan region is very low. Environmental and management factors are the major causes, which influence the regional prevalence.
Bjerkas I, Mohn SF, Presthus J. Unidentified cyst-forming sporozoon causing encephalomyelitis and myositis in dogs. Zeitschrift fur Parasitenkunde (Berlin, Germany). 1984;70(2):271-4.
Dubey J. Neosporosis in cattle: Biology and economic impact. J. Am. Vet. Med. Assoc. 1999;214(8):1160.
Anderson M, Andrianarivo A, Conrad P. Neosporosis in cattle. Anim. Reprod. Sci. 2000;60:417-31.
Gharekhani J, Tavoosidana G, Akbarein H. Serological study of Neospora caninum infection in dogs and cattle from west of Iran. Comp. Clin. Pathol. 2014;23(5):1203-7.
Barling KS, McNeill JW, Thompson JA, Paschal JC, McCollum III FT, Craig TM, et al. Association of serologic status for Neospora caninum with postweaning weight gain and carcass measurements in beef calves. J. Am. Vet. Med. Assoc. 2000;217(9):1356-60.
Dubey J. Recent advances in Neospora and neosporosis. Vet. Parasitol.1999;84(3):349-67.
Waldner CL, Janzen E, Ribble C. Determination of the association between Neospora caninum infection and reproductive performance in beef herds. J. Am. Vet. Med. Assoc. 1998;213(5):685-90.
Björkman C, Uggla A. Serological diagnosis of Neospora caninum infection. Int. J. Parasitol. 1999;29(10):1497-507.
Dubey J, Lindsay D. A review of Neospora caninum and neosporosis. Vet. Parasitol. 1996;67(1-2):1-59.
Dubey J, Schares G. Neosporosis in animals—the last five years. Vet. Parasitol. 2011;180(1):90-108.
Dubey J, Schares G, Ortega-Mora L. Epidemiology and control of neosporosis and Neospora caninum. Clin. Microbiol. Rev. 2007;20(2):323-67.
Innes EA, Wright S, Bartley P, Maley S, Macaldowie C, Esteban-Redondo I, et al. The host–parasite relationship in bovine neosporosis. Vet. Immunol. Immunopathol. 2005;108(1):29-36.
Hajikolaei MH, Hamidinejat H, Ghorbanpoor M, Goraninejad S. Serological study of Neospora caninum infection in cattle from Ahvaz area, Iran. Int. J. Vet. Res. 2008;2(1):63-127.
Ranjbar-Bahadori. Sh. MAH, Bokai. S. , Yosefi. M.R. . Serological study of Neospora caninum in aborted dairy cattle in Garmsar. Journal of comparative pathobiology. 2010(2):249-54.[In persian]
Reza Nourollahi Fard S, Khalili M, Aminzadeh A. Prevalence of antibodies to Neospora caninum in cattle in Kerman province, South East Iran. Vet. Arh. 2008;78(3):253-9.
Sadrebazzaz A, Haddadzadeh H, Esmailnia K, Habibi G, Vojgani M, Hashemifesharaki R. Serological prevalence of Neospora caninum in healthy and aborted dairy cattle in Mashhad, Iran. Vet. Parasitol. 2004;124(3):201-4.
Haddad JPA, Dohoo IR, VanLeewen JA. A review of Neospora caninum in dairy and beef cattle—a Canadian perspective. Can. Vet. J. 2005;46(3):230.
Moen AR, Wouda W, Mul MF, Graat EAM, van Werven T. Increased risk of abortion following neospora caninum abortion outbreaks: a retrospective and prospective cohort study in four dairy herds. Theriogenology. 1998;49(7):1301-9.
Thurmond MC, Hietala SK. Effect of congenitally acquired Neospora caninum infection on risk of abortion and subsequent abortions in dairy cattle. Am. J. Vet. Res. 1997;58(12):1381-5.
Salehi N, Haddadzadeh H, Shayan P, Vodjgani M, Bolourchi M. Serological study of Neospora caninum in pregnant dairy cattle in Tehran, Iran. Int. J. Vet. Res. 2010;4(2):113-35.
Ansari-Lari M, Rowshan-Ghasrodashti A, Jesmani H, Masoudian M, Badkoobeh M, editors. Association of Neospora caninum with reproductive performance in dairy cows: A prospective study from Iran. Vet Res Forum; 2017; 8(2): 101-106
Heidari H, Mohammadzadeh A, Gharekhani J. Seroprevalence of Neospora caninum in slaughtered native cattle in Kurdistan province, Iran. Vet Res Forum: an International Quarterly Journal. 2014;5(1):69-72.
Schares G, Conraths F, Reichel M. Bovine neosporosis: comparison of serological methods using outbreak sera from a dairy herd in New Zealand. Int. J. Parasitol. 1999;29(10):1659-67.
Venturini M, Venturini L, Bacigalupe D, Machuca M, Echaide I, Basso W, et al. Neospora caninum infections in bovine foetuses and dairy cows with abortions in Argentina. Int. J. Parasitol. 1999;29(10):1705-8.
Kyaw T, Virakul P, Muangyai M, Suwimonteerabutr J. Neospora caninum seroprevalence in dairy cattle in central Thailand. Vet. Parasitol. 2004;121(3):255-63.
Fujii T, Kasai N, Nishi S, Dubey J, Gennari S. Seroprevalence of Neospora caninum in female water buffaloes (Bubalus bubalis) from the southeastern region of Brazil. Vet. Parasitol. 2001;99(4):331-4.
Sanderson M, Gay J, Baszler T. Neospora caninum seroprevalence and associated risk factors in beef cattle in the northwestern United States. Vet. Parasitol. 2000;90(1):15-24.
Guimaraes JS, Jr., Souza SL, Bergamaschi DP, Gennari SM. Prevalence of Neospora caninum antibodies and factors associated with their presence in dairy cattle of the north of Parana state, Brazil. Vet. Parasitol. 2004;124(1-2):1-8.
Moore D. Neosporosis in South America. Vet. Parasitol. 2005;127(2):87-97.