Iranian Journal of Parasitology 2017. 12(2):188-195.

M-RNA Gene Expression of INF-Γ and IL-10 during Intestinal Phase of Trichinella spiralis after Myrrh and Albendazole Treatment
Hanaa Y. BAKIR, Rasha AH ATTIA, Abeer E MAHMOUD, Zedan IBRAHEIM

Abstract


Background: The protective response developed against Trichinella spiralis infection provokes immune and inflammatory responses mediated by cytokines released from T helper cells. We aimed to evaluate the effect of albendazole or myrrh on the expression of IFN-γ and IL-10 in BALB/c mice infected with T. spiralis.

Methods: This study was done at the Animal House of Faculty of Medicine, Assiut University (Assiut, Egypt) from April to December 2015. Mice were infected with 300 T. spiralis larvae and treated with albendazole (50 mg/kg per day) or myrrh (500 mg/kg per day) for 3 consecutive days post-infection (pi). The expression of INF-γ and IL-10 was detected in the intestinal tissue by reverse transcription (RT) PCR.

Results: The expression of IFN-γ in mice treated with albendazole and myrrh was detected on days 3 and 15 pi respectively. In the control group, it was found on days 5, 10, 15 and 20 pi with the highest expression on day 15 pi. The expression of IL-10 was detected on days 3, 20 pi in the albendazole and myrrh treated groups, respectively. In the control group, IL-10 expression appeared on days 5 and day 20 pi.

Conclusion: The target of albendazole and myrrh on the profile of IFN-γ and IL-10 on these cytokines were encouraging to reinforce their therapeutic use against trichinellosis.

Keywords


Trichinella spiralis; Albendazole; Myrrh; IFN-γ; IL-10

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References


Morales MA, Mele R, Sanchez M, Sacchini D, De Giacomo M, Pozio E. Increased CD8+T cell expression and a type 2 cytokine pattern during the muscular phase of Trichinella Infection in humans. Infect Immun. 2002;70:233-9.

Li CKF, Seth R, Gray T, Bayston R, Mahida YR, Wakelin D. Production of pro-inflammatory cytokines and inflammatory mediators in human intestinal epithelial cells after invasion by Trichinella spiralis. Infect Im-mun.1998;66(5):2200–6.

Helmby H, Grencis RK. Contrasting roles for IL-10 in protective immunity to different life cycle stages of intestinal nematode parasites. Eur J Immunol. 2003; 33(9):2382-90.

Bliss SK, Alcaraz A, Appleton JA. IL-10 prevents liver necrosis during murine infection with Trichinella spiralis. J Immunol. 2003; 171(6):3142-7.

Bliss SK, Bliss SP, Beiting DP, Alcaraz A, Appleton JA. IL-10 regulates movement of intestinally derived CD4+ T cells to the liver. J Immunol. 2007; 178 (12) 7974-7983.

Yang Y, Wang Y, Zhan B, Gu Y, Cheng Y, Zhu X. Excretory/secretory products from Trichinella spiralis adult worms ameliorate DSS-induced colitis in mice. PLos One. 2014;9(5): e96454.

Chung MS, Joo KH, Quan FS, Kwon HS, Cho SW. Efficacy of flubendazole and albendazole against Trichinella spiralis in mice. Parasite. 2001;8 (2 Suppl):S195-8.

Siriyasatien P, Yingyourd P, Nuchprayoon S. Efficacy of albendazole against early and late stage of Trichinella spiralis infection in mice. J Med Assoc Thai. 2003;86 Suppl 2 :S257-62.

Shoheib ZS, Shamloula MM, Abdin AA, El-Segai O. Role of α-chymotrypsin and colchicine as adjuvant therapy in experimental muscular trichinellosis: parasitological, biochemical and immunohistochemical study. Egypt J Med Microbiol. 2006;15:773-790.

Shalaby MA, Moghazy FM, Shalaby HA, Nasr SM. Effect of methanolic extract of Balanites aegyptiaca fruits on enteral and parenteral stages of Trichinella spiralis in rats. Parasitol Res. 2010;107:17-25.

Attia RAH, Mahmoud AE, Farrag HM, Makboul R, Mohamed ME, Ibraheim Z. Effect of myrrh and thyme on Trichinella spiralis enteral and parenteral phases with inducible nitric oxide expression in mice. Mem Inst Oswaldo Cruz. 2015;110:1035-1041.

Zeromski J, Boczoń K, Wandurska-Nowak E, Mozer-Lisewska I. Effect of aminoguanidine and albendazole on inducible nitric oxide synthase (iNOS) activity in T. spiralis-infected mice muscles. Folia Histochem Cytobiol. 2005;43:157-9.

Wojtkowiak-Giera A, Wandurska-Nowak E, Michalak M, Derda M, Lopaciuch J. Trichinellosis in mice: effect of albendazole on the glutathione transferase in the intestines. Folia Parasitol (Praha). 2012;59:311-4.

Chen KM, Lai SC. Biochemical and pathological evaluation of albendazole/thalidomide co-therapy against eosinophilic meningitis or enin-goencephalitis induced by Angiostrongylus cantonensis. J Antimicrob Chemother. 2007;59:264–276.

Cooper PJ, Moncayo AL, Guadalupe I, Benitez S, Vaca M, Chico M, Griffin GE. Repeated treatments with albendazole enhance Th2 responses to Ascaris lumbricoides but not aeroallergens in children from rural communities in the Tropics. J Infect Dis. 2008;198:1237–1242.

Diao Z, Chen X, Yin C, Wang J, Qi H, Ji A. Angiostrongylus cantonensis: effect of combination therapy with albendazole and dexamethasone on Th cytokine gene expression in PBMC from patient with eosinophilic meningitis. Exp Parasitol. 2009;123:1-5.

Blish CA, Sangaré L, Herrin BR, Richardson BA, John-Stewart G, Walson JL. Changes in plasma cytokines following treatment of Ascaris lumbricoides in HIV-1 infected individuals. J Infect Dis. 2010;201:1816–1821.

Jin-Fu Y, Jie Y, Yuan L. Influence of albendazole combined with astragalus injection on Th1/Th2 cytokines of patients infected with Clonorchis sinensis. Chinese General Practice. 2013;33.

Dolara P, Corte B, Ghelardini C, Pugliese AM, Cerbai E, Menichetti S, Lo Nostro A. Local anaesthetic and antifungal proper¬ties of sesquiterpenes from myrrh. Planta Med. 2000;66: 356-358.

Basyoni MM, El-Sabaa AA. Therapeutic potential of myrrh and ivermectin against experimental Trichinella spiralis infection in mice. Korean J Parasitol. 2013;51:297-304.

Ahmad A, Raish M, Ganaie MA, et al. Hepatoprotective effect of Commiphora myrrha against d-GalN/LPS-induced hepatic injury in a rat model through attenuation of pro-inflammatory cytokines and related genes. Pharm Biol. 2015;53:1759-67.

Su S, Duan J, Chen T, et al. Corrigendum: Frankincense and myrrh suppress inflammation via regulation of the metabolic profiling and the MAPK signaling pathway. Sci Rep. 2015; 5:15597.

Gamble HR. Detection of trichinellosis in pigs by artificial digestion and enzyme immunoassay. J Food Prot. 1996; 59:295-8.

Evans WC. Trease, Evans’ Pharmacognosy, 15th ed., Elsevier, Edinburgh, 2002;585.

Repetto G, Del Peso A, Zurita JL. Neutral red uptake assay for the estimation of cell viability/cytotoxicity. Nat Protoc. 2008; 3:1125-1131.

EDQM Council of Europe. 2014 European Pharmacopoeia. edqm.eu/en/european-pharmacopoeia-8th-edition-1563.html.

Hussaini SN, Hassanali HT. Limulus amoebocyte lysate assay of endotoxin: a method for visual detection of the positive gel reaction. J Med Microbiol. 1987; 24:89-90.

Picherot M, Oswald IP, Cote M, et al. Swine infection with Trichinella spiralis: Comparative analysis of the mucosal intestinal and systemic immune responses. Vet Parasitol. 2007; 143:122–30.

Despommier DD. Trichinella spiralis and the concept of niche. J Parasitol. 1993;79:472- 482.

Dozois CM, Oswald E, Gautier N, et al. A reverse transcription-polymerase chain reaction method to analyze porcine cytokine gene expression. Vet Immunol Immunopathol. 1997;58:287–300.

Moreira DL, Teixeira SS, Monteiro MH, et al. Traditional use and safety of herbal medicines. Rev Bras Farmacogn. 2014; 24:248-257.

Christ AD, Blumberg RS. The intestinal epithelial cells: immunological aspects. Springer Semin Immunopathol. 1997; 18:449-461.

Khan WI, Vallance BA, Blennerhassett PA, et al. Critical role for signal transducer and activator of transcription factor 6 in mediating intestinal muscle hypercontractility and worm expulsion in Trichinella spiralis-infected mice. Infect Immun. 2001; 69:838-44.

Kang SA, Cho MK, Park MK, et al. Alteration of helper T-cell related cytokine production in splenocytes during Trichinella spiralis infection. Vet Parasitol. 2012; 186:319-27.

Kim S, Park MK, Yu HS. Toll-Like receptor gene expression during Trichinella spiralis infection. Korean J Parasitol. 2015; 53:431-8.


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