Molecular and Morphological Characterizations of Echinococcus granulosus from Human and Animal Isolates in Kashan, Markazi Province, Iran

  • Mohsen ARBABI Dept. of Medical Parasitology and Mycology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran
  • Majid PIRESTANI Dept. of Medical Parasitology and Entomology, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran
  • Mahdi DELAVARI Dept. of Medical Parasitology and Mycology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran
  • Hossein HOOSHYAR Dept. of Medical Parasitology and Mycology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran
  • Amir ABDOLI Dept. of Medical Parasitology and Entomology, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran
  • Shahab SARVI Dept. of Medical Parasitology and Entomology, Faculty of Medicine, Mazandaran University, Sari, Iran
Keywords: Echinococcus granulosus, Molecular phylogenetics, Morphological characteriza-tion, PCR-RFLP, Iran

Abstract

Background: One of the most important zoonotic helminths in the world is known as Echinococcus granulosus. Different strains of the E. granulosus have been described based on morphological and molecular characterizations, however, there is limited information regarding the characteristics of the phenotypes and genotypes of E. granulosus in Iran.Methods: The present study was prepared to evaluate the phenotypic and genotypic diversity of E. granulosus isolates collected from human, goat, sheep, and cattle based on 19 standard morphometric parameters and mitochondrial and nuclear genes (CO1, ND1, and ITS1) in Kashan, Markazi Province, Iran during 2013-2014.Results: The biometric analysis for the 19 characters revealed that the 19 morphometric values of cattle isolates were exceptionally higher than human, goat, and sheep isolates (P<0.05). Molecular analysis confirms the morphological findings. Phylogenic analysis of the CO1, NAD1 and ITS1 genes for all isolates, independent of the host, revealed that the common sheep strain (G1) is traveling among livestock in Kashan and the strains are highly adapted to goats, cattle, sheep, and humans.Conclusion: Both morphological and molecular results of this study indicated that the only genotype G1 of E. granulosus travels between humans and other intermediate hosts of this parasite in the area study.

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Published
2017-06-13
How to Cite
1.
ARBABI M, PIRESTANI M, DELAVARI M, HOOSHYAR H, ABDOLI A, SARVI S. Molecular and Morphological Characterizations of Echinococcus granulosus from Human and Animal Isolates in Kashan, Markazi Province, Iran. IJPA. 12(2):177-8.
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