Iranian Journal of Parasitology 2016. 11(4):499-506.

Microscopic and Molecular Detection of Cryptosporidium andersoni and Cryptosporidium xiaoi in Wastewater Samples of Tehran Prov-ince, Iran
Kareem HATAM-NAHAVANDI, Mehdi MOHEBALI, Amir-Hossein MAHVI, Hossein KESHAVARZ, Hamid-Reza NAJAFIAN, Hamed MIRJALALI, Sasan REZAEI, Mostafa REZAEIAN

Abstract


Background: As a waterborne pathogen, Cryptosporidium is one of the most common causes of gastroenteritis in human and hoofed livestock animals. This study aimed to investigate the distribution of Cryptosporidium spp. in human and livestock wastewaters in Iran, by the 18S rRNA sequence analysis.

Methods: A total of 54 raw wastewater samples collected from three urban treatment plants and two slaughterhouses during 2014-2015 in Tehran, Iran. The presence of the Cryptosporidium oocysts was assessed by immunofluorescence with monoclonal antibodies. To characterize the oocysts at the molecular level, the 18S rRNA gene of Cryptosporidium was PCR amplified and sequenced.

Results: Of the 54 wastewater samples examined, 34 (62.9%) were positive for Cryptosporidium oocysts using the IFA. Of these, 70.5% (24/34) were positive by PCR, that 91.6% (22/24) were successfully sequenced. The species of C. andersoni (95.4%) and C. xiaoi (4.6%) were detected in livestock wastewater samples.

Conclusion: C. andersoni was the major Cryptosporidium sp. found in the aquatic environmental wastewater samples. The high rate of detection of C. andersoni in domestic wastewater was probably the result of the predominancy of this species in cattle herds in Iran. The current study is the first report of C. xiaoi in Iran.

Keywords


Cryptosporidium andersoni; Cryptosporidium xiaoi; Iran; Wastewater

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References


Fletcher SM, Stark D, Harkness J, Ellis J. Enteric protozoa in the developed world: a public health perspective. Clin Microbiol Rev. 2012; 25(3):420-49.

Karanis P, Kourenti C, Smith H. Waterborne transmission of protozoan parasites: a worldwide review of outbreaks and lessons learnt. J Water Health, 2007; 5(1):1-38.

Feng Y, Zhao X, Chen J, Jin W, Zhou X, Li N, Wang L, Xiao L. Occurrence, source, and human infection potential of Cryptosporidium and Giardia spp. in source and tap water in shanghai, china. Appl Environ Microbiol. 2011; 77(11):3609-16.

Xiao L, Sulaiman IM, Ryan UM, Zhou L, Atwill ER, Tischler ML, Xhang X, Fayer R, Lal AA. Host adaptation and host-parasite co-evolution in Cryptosporidium: implications for taxonomy and public health. Int J Parasitol. 2002; 32(14):1773-85.

Chalmers RM, Katzer F. Looking for Cryptosporidium: the application of advances in detection and diagnosis. Trends Parasitol. 2013; 29(5):237-51.

Leoni F, Amar C, Nichols G, Pedraza-Diaz S, McLauchlin J. Genetic analysis of Cryptosporidium from 2414 humans with diarrhoea in England between 1985 and 2000. J Med Microbiol. 2006; 55(6):703-7.

Santin M, Trout JM, Xiao L, Zhou L, Greiner E, Fayer R. Prevalence and age-related variation of Cryptosporidium species and genotypes in dairy calves. Vet Parasitol. 2004; 122(2):103-17.

Mirzai Y, Yakhchali M, Mardani K. Cryptosporidium parvum and Cryptosporidium andersoni infection in naturally infected cattle of northwest Iran. Vet Res Forum, 2014; 5(1):55-60.

Pirestani M, Sadraei J, Dalimi Asl A, Zavvar M, Vaeznia H. Molecular characterization of Cryptosporidium isolates from human and bovine using 18s rRNA gene in Shahriar county of Tehran, Iran. Parasitol Res. 2008; 103(2):467-72.

Meamar AR, Guyot K, Certad G, Dei-Cas E, Mohraz M, Mohebali M, Mohammad K, Mehbod AA, Rezaie S, Rezaian M. Molecular characterization of Cryptosporidium isolates from humans and animals in Iran. Appl Environ Microbiol. 2007; 73(3):1033-5.

Mahmoudi M, Ashrafi K, Abedinzadeh H, Tahvildar-Bideruni F, Haghighi A, Bandehpour M, Taghipour Lailabadi N, Kazemi B. Development of sensitive detection of Cryptosporidium and Giardia from surface water in Iran. Iran J Parasitol. 2011; 6(3):43-51.

Mahmoudi MR, Kazemi B, Mohammadiha A, Mirzaei A, Karanis P. Detection of Cryptosporidium and Giardia (oo)cysts by IFA, PCR and LAMP in surface water from Rasht, Iran. Trans R So Trop Med Hyg. 2013; 107(8):511-7.

Hatam-Nahavandi K, Mahvi AH, Mohebali M, Keshavarz H, Mobedi I, Rezaeian M. Detection of parasitic particles in domestic and urban wastewaters and assessment of removal efficiency of treatment plants in Tehran, Iran. J Environ Health Sci Eng. 2015; 13:4.

Hatam-Nahavandi K, Mohebali M, Mahvi A-H, Keshavarz H, Khanaliha K, Tarighi F, Molaei-Rad MB, Rezaeian T, Charehdar S, Salimi M, Farnia S, Rezaeian M. Evaluation of Cryptosporidium oocyst and Giardia cyst removal efficiency from urban and slaughterhouse wastewater treatment plants and assessment of cyst viability in wastewater effluent samples from Tehran, Iran. J Water Reuse Desalination. 2015; 5(3):372-90.

Nahavandi KH, Mahvi AH, Mohebali M, Keshavarz H, Rezaei S, Mirjalali H, Elikaei S, Rezaeian M. Molecular Typing of Eimeria ahsata and Eimeria crandallis Isolated From Slaughterhouse Wastewater. J J Microbiol. 2016; 9(4): e34140. (In press).

Hatam-Nahavandi K, Mohebali M, Mahvi A-H, Keshavarz H, Mirjalali H, Rezaei S, Meamar AR, Rezaeian M. Subtype analysis of Giardia duodenalis isolates from municipal and domestic raw wastewaters in Iran. Environ Sci Pollut Res. 2016; 1-8.

Jiang J, Alderisio KA, Xiao L. Distribution of Cryptosporidium genotypes in storm event water samples from three watersheds in New York. Appl Environ Microbiol. 2005; 71(8):4446-54.

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol. 2013; 30(12):2725-9.

Caccio SM, De Giacomo M, Aulicino FA, Pozio E. Giardia cysts in wastewater treatment plants in Italy. Appl Environ Microbiol. 2003; 69(6):3393-8.

Kitajima M, Haramoto E, Iker BC, Gerba CP. Occurrence of Cryptosporidium, Giardia, and Cyclospora in influent and effluent water at wastewater treatment plants in Arizona. Sci Total Environ. 2014; 484:129-36.

Xiao L, Singh A, Limor J, Graczyk TK, Gradus S, Lal A. Molecular characterization of Cryptosporidium oocysts in samples of raw surface water and wastewater. Appl Environ Microbiol. 2001; 67(3):1097-101.

Koyama Y, Satoh M, Maekawa K, Hikosaka K, Nakai Y. Isolation of Cryptosporidium andersoni Kawatabi type in a slaughterhouse in the northern island of Japan. Vet Parasitol. 2005; 130(3-4):323-6.

Ayed LB, Yang W, Widmer G, Cama V, Ortega Y, Xiao L. Survey and genetic characterization of wastewater in Tunisia for Cryptosporidium spp., Giardia duodenalis, Enterocytozoon bieneusi, Cyclospora cayetanensis and Eimeria spp. J Water Health. 2012; 10(3):431-44.

Fallah E, Mahdavi Poor B, Jamali R, Hatam-Nahavandi K, Asgharzadeh M. Molecular characterization of Cryptosporidium isolates from cattle in a slaughterhouse in Tabriz, northwestern Iran. J Biol Sci. 2008; 8(3):639-43.

Fayer R, Santin M. Cryptosporidium xiaoi n. sp. (Apicomplexa: Cryptosporidiidae) in sheep (Ovis aries). Vet Parasitol. 2009; 164(2-4):192-200.


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